Anti-Ferritin Heavy Chain抗体(ab65080)

概述

  • 产品名称Anti-Ferritin Heavy Chain抗体
    参阅全部 Ferritin Heavy Chain 一抗
  • 描述
    兔多克隆抗体to Ferritin Heavy Chain
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Dog, Human
    预测可用于: Sheep, Goat, Cow, Cat, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Ferritin Heavy Chain aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P02794
    (Peptide available as ab90631)

  • 阳性对照
    • This antibody gave a positive signal in Human Liver Tissue Lysate. IF/ICC: MCF7 cell line. IHC-P: FFPE human normal liver tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab65080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-Ferritin Heavy Chain antibody 图像

  • Anti-Ferritin Heavy Chain antibody (ab65080) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 24 kDa (possible post-translational modification).

    Exposure time : 3 minutes
    Ferritin Heavy Chain contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a slightly higher than predicted molecular weight (24 kDa).
  • IHC image of ab65080 staining in Human Normal Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65080, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab65080 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65080, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-Ferritin Heavy Chain antibody (ab65080) at 1 µg/ml

    Lane 1 : Recombinant Human Ferritin Heavy Chain protein (ab78877) at 0.1 µg
    Lane 2 : Recombinant Human Ferritin Heavy Chain protein (ab78877) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa


    Exposure time : 30 seconds

Anti-Ferritin Heavy Chain antibody (ab65080)参考文献

This product has been referenced in:
  • Pereira SM  et al. Overexpression of the MRI Reporter Genes Ferritin and Transferrin Receptor Affect Iron Homeostasis and Produce Limited Contrast in Mesenchymal Stem Cells. Int J Mol Sci 16:15481-96 (2015). WB ; Mouse . Read more (PubMed: 26184159) »
  • Li W  et al. Systemic and cerebral iron homeostasis in ferritin knock-out mice. PLoS One 10:e0117435 (2015). WB, IHC-P ; Mouse . Read more (PubMed: 25629408) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse Retina)
Permeabilization No
Specification Mouse Retina
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Aug 03 2016

Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
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Abcam user community

Verified customer

提交于 Sep 09 2015

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The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse and human samples . If we cannot resolve the issue you are having with the antibody then I would be ...

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