Anti-Ferritin抗体[F23] (ab10060)


  • 产品名称Anti-Ferritin抗体[F23]
    参阅全部 Ferritin 一抗
  • 描述
    小鼠单克隆抗体[F23] to Ferritin
  • 经测试应用适用于: ICC/IF, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Ferritin. Purified human ferritin
    Database link: P02794

  • 常规说明Concentration varies from lot to lot and can be provided on request.




Our Abpromise guarantee covers the use of ab10060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [F31] to Ferritin (HRP) (ab24475). Can be used as Capture antibody.


  • 功能Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • 序列相似性Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • 数据库链接
  • 形式Subunit structure: Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.
  • 别名
    • Cell proliferation-inducing gene 15 protein antibody
    • Ferritin H subunit antibody
    • Ferritin heavy chain antibody
    • Ferritin heavy polypeptide 1 antibody
    • Ferritin L subunit antibody
    • Ferritin light polypeptide antibody
    • Ferritin, heavy polypeptide antibody
    • FRIH_HUMAN antibody
    • FTH antibody
    • FTH1 antibody
    • FTL antibody
    see all

Anti-Ferritin antibody [F23] 图像

  • ICC/IF image of ab10060 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10060, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ferritin antibody [F23] (ab10060)参考文献

ab10060 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

I contacted the source the two antibodies and got the following information:

ab10060 :

This antibody has been tested to work at least with human serum ferritin and liver ferritin.It was not test...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 20 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Jun 02 2009

Thank you for your enquiry. I have been in touch with the source of our ferritin antibodies and I can tell you that from the feedback that we have received from our customers both monoclonal antibodies; ab10060 and ab24475 are specific to the L-subu...

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