重组
RabMAb

Anti-Ferritin抗体[EPR3004Y] (ab75973)

概述

  • 产品名称
    Anti-Ferritin抗体[EPR3004Y]
    参阅全部 Ferritin 一抗
  • 描述
    兔单克隆抗体[EPR3004Y] to Ferritin
  • 特异性
    This antibody recognizes both, Ferritin heavy and light chains.
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Frmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Ferritin aa 50-150. The exact sequence is proprietary.
    Database link: P02794

  • 阳性对照
    • WB: Jurkat, SH-SY-5Y, C6, Raw264.7, human and rat brain lysates ICC/IF: Jurkat cells
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab75973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/1000 - 1/2000. Detects a band of approximately 19, 21 kDa (predicted molecular weight: 21,19 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 25498860
  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能
      Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
    • 序列相似性
      Belongs to the ferritin family.
      Contains 1 ferritin-like diiron domain.
    • Information by UniProt
    • 数据库链接
    • 形式
      Subunit structure: Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.
    • 别名
      • Cell proliferation-inducing gene 15 protein antibody
      • Ferritin H subunit antibody
      • Ferritin heavy chain antibody
      • Ferritin heavy polypeptide 1 antibody
      • Ferritin L subunit antibody
      • Ferritin light polypeptide antibody
      • Ferritin, heavy polypeptide antibody
      • FRIH_HUMAN antibody
      • FTH antibody
      • FTH1 antibody
      • FTL antibody
      see all

    图片

    • Immunofluorescence staining of Jurkat cells with purified ab75973 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75973 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • All lanes : Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/2000 dilution (purified)

      Lane 1 : Rat brain lysate
      Lane 2 : C6 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 21,19 kDa
      Observed band size : 21 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/10000 dilution (purified) + Raw264.7 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 21,19 kDa
      Observed band size : 21 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/10000 dilution (purified)

      Lane 1 : SH-SY5Y whole cell lysate
      Lane 2 : Jurkat whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 21,19 kDa
      Observed band size : 21 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/2000 dilution (Unpurified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : SH-SY-5Y cell lysate
      Lane 3 : Human brain lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 21,19 kDa
      Observed band size : 19,21 kDa

    文献

    This product has been referenced in:
    • Rychtarcikova Z  et al. Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism. Oncotarget 8:6376-6398 (2017). WB ; Human . Read more (PubMed: 28031527) »
    • Al-Eisawi Z  et al. Novel Mechanism of Cytotoxicity for the Selective Selenosemicarbazone, 2-Acetylpyridine 4,4-Dimethyl-3-selenosemicarbazone (Ap44mSe): Lysosomal Membrane Permeabilization. J Med Chem 59:294-312 (2016). Read more (PubMed: 26645570) »

    See all 17 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (Eye, muscle)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gel, NuPAGE)
    Loading amount
    23 µg
    Specification
    Eye, muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Cheri Ackerman

    Verified customer

    提交于 Aug 11 2016

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (TGX any size gel)
    Sample
    Fish Cell lysate - whole cell (Liver)
    Specification
    Liver
    Blocking step
    (agent) for 20 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Username

    Don Kuhn

    Verified customer

    提交于 Oct 29 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Loading amount
    20 µg
    Specification
    liver
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 06 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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