兔多克隆抗体to FER (phospho Y402)
ab79573 detects endogenous levels of FER only when phosphorylated at tyrosine 402.
Synthetic phosphopeptide derived from human FER around the phosphorylation site of tyrosine 402 (V-N-Y
Human brain tissue; Jurkat cell extracts starved for 24 hours.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab79573 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 93 kDa.
1/50 - 1/100.
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
Expressed in a variety of lymphoid cell lines.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.
Cytoplasm. Nucleus. Associated with the chromatin.
Information by UniProt
c FER antibody
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Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER (phospho Y402) antibody (ab79573)
ab79573, at a 1/50 dilution, staining FER in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
Western blot - FER (phospho Y402) antibody (ab79573)
All lanes : Anti-FER (phospho Y402) antibody (ab79573) at 1/500 dilution Lane 1 : Jurkat cell extracts, starved (24 hours) Lane 2 : Jurkat cell extracts, starved (24 hours) with immunizing phospho peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size: 93 kDa Observed band size: 93 kDa Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
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