概述

  • 产品名称Anti-FER (phospho Y402)抗体
    参阅全部 FER 一抗
  • 描述
    兔多克隆抗体to FER (phospho Y402)
  • 特异性ab79573 detects endogenous levels of FER only when phosphorylated at tyrosine 402.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human FER around the phosphorylation site of tyrosine 402 (V-N-YP-E-E)

  • 阳性对照
    • Human brain tissue; Jurkat cell extracts starved for 24 hours.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab79573 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 93 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
  • 组织特异性Expressed in a variety of lymphoid cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
    Contains 1 FCH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • 细胞定位Cytoplasm. Nucleus. Associated with the chromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c FER antibody
    • Feline encephalitis virus related kinase FER antibody
    • Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
    • Fer (fps/fes related) tyrosine kinase antibody
    • FER antibody
    • FER_HUMAN antibody
    • FerT antibody
    • Fujinami poultry sarcoma/Feline sarcoma related protein Fer antibody
    • p94 FER antibody
    • p94-FER antibody
    • Pe1Fe10 antibody
    • Pe1Fe13 antibody
    • Pe1Fe3 antibody
    • Pe1Fe6 antibody
    • Phosphoprotein NCP94 antibody
    • PPP1R74 antibody
    • Protein phosphatase 1 regulatory subunit 74 antibody
    • Proto oncogene tyrosine protein kinase FER antibody
    • Proto-oncogene c-Fer antibody
    • TYK3 antibody
    • Tyrosine kinase 3 antibody
    • Tyrosine-protein kinase Fer antibody
    see all

Anti-FER (phospho Y402) antibody 图像

  • ab79573, at a 1/50 dilution, staining FER in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
  • All lanes : Anti-FER (phospho Y402) antibody (ab79573) at 1/500 dilution

    Lane 1 : Jurkat cell extracts, starved (24 hours)
    Lane 2 : Jurkat cell extracts, starved (24 hours) with immunizing phospho peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

Anti-FER (phospho Y402) antibody (ab79573)参考文献

This product has been referenced in:
  • Chang SF  et al. Different modes of endothelial-smooth muscle cell interaction elicit differential ß-catenin phosphorylations and endothelial functions. Proc Natl Acad Sci U S A 111:1855-60 (2014). Read more (PubMed: 24449884) »

See 1 Publication for this product

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