概述

  • 产品名称Anti-FER抗体[EP1842Y]
    参阅全部 FER 一抗
  • 描述
    兔单克隆抗体[EP1842Y] to FER
  • 特异性This antibody reacts with FER
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the N terminus of FER (Human)

  • 阳性对照
    • Jurkat cell lysate
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab52479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
IP Use a concentration of 5 µg/ml.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
    • 组织特异性Expressed in a variety of lymphoid cell lines.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
      Contains 1 FCH domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
    • 细胞定位Cytoplasm. Nucleus. Associated with the chromatin.
    • Information by UniProt
    • 数据库链接
    • 别名
      • c FER antibody
      • Feline encephalitis virus related kinase FER antibody
      • Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
      • Fer (fps/fes related) tyrosine kinase antibody
      • FER antibody
      • FER_HUMAN antibody
      • FerT antibody
      • Fujinami poultry sarcoma/Feline sarcoma related protein Fer antibody
      • p94 FER antibody
      • p94-FER antibody
      • Pe1Fe10 antibody
      • Pe1Fe13 antibody
      • Pe1Fe3 antibody
      • Pe1Fe6 antibody
      • Phosphoprotein NCP94 antibody
      • PPP1R74 antibody
      • Protein phosphatase 1 regulatory subunit 74 antibody
      • Proto oncogene tyrosine protein kinase FER antibody
      • Proto-oncogene c-Fer antibody
      • TYK3 antibody
      • Tyrosine kinase 3 antibody
      • Tyrosine-protein kinase Fer antibody
      see all

    Anti-FER antibody [EP1842Y] 图像



    • Predicted band size : 93 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: FER knockout HAP1 cell lysate (40 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab52479 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

    • Anti-FER antibody [EP1842Y] (ab52479) at 1/5000 dilution + Jurkat cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 93 kDa
      Observed band size : 93 kDa
    • Overlay histogram showing Jurkat cells stained with ab52479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52479, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • FER was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to FER and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52479.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 93kDa; FER

    Anti-FER antibody [EP1842Y] (ab52479)参考文献

    This product has been referenced in:
    • McGinnis LK  et al. Fer tyrosine kinase is required for germinal vesicle breakdown and meiosis-I in mouse oocytes. Mol Reprod Dev 78:33-47 (2011). IHC-P, IF ; Mouse . Read more (PubMed: 21268181) »
    • Whitehead SN  et al. Transient and bilateral increase in Neuropilin-1, Fer kinase and collapsin response mediator proteins within membrane rafts following unilateral occlusion of the middle cerebral artery in mouse. Brain Res 1344:209-16 (2010). WB ; Mouse . Read more (PubMed: 20493826) »

    See all 3 Publications for this product

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