重组Anti-FER抗体[EP1842Y] (ab52479)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1842Y] to FER
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-FER抗体[EP1842Y]
参阅全部 FER 一抗 -
描述
兔单克隆抗体[EP1842Y] to FER -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human FER (N terminal). The exact sequence is proprietary.
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阳性对照
- WB: HeLa, Jurkat, PC-12, and NIH/3T3 whole cell lysates.
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常规说明
This product has switched from a hybridoma to recombinant production method on 4th September 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1842Y -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52479于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa). |
靶标
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功能
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control. -
组织特异性
Expressed in a variety of lymphoid cell lines. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
细胞定位
Cytoplasm. Nucleus. Associated with the chromatin. - Information by UniProt
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数据库链接
- Entrez Gene: 2241 Human
- Entrez Gene: 14158 Mouse
- Omim: 176942 Human
- SwissProt: P16591 Human
- SwissProt: P70451 Mouse
- SwissProt: P09760 Rat
- Unigene: 221472 Human
- Unigene: 23039 Mouse
see all -
别名
- c FER antibody
- Feline encephalitis virus related kinase FER antibody
- Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
see all
图片
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All lanes : Anti-FER antibody [EP1842Y] (ab52479) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa -
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: FER knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab52479 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.ab52479 was shown to specifically react with FER when FER knockout samples were used. Wild-type and FER knockout samples were subjected to SDS-PAGE. Ab52479 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-FER antibody [EP1842Y] (ab52479) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FER knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab52479 observed at 100 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab52479 was shown to react with FER in wild-type HeLa cells in Western blot with loss of signal observed in FER knockout cell line ab265226 (FER knockout cell lysate ab257950). Wild-type and FER knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab52479 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab52479 被引用在 4 文献中.
- Hu X et al. Downregulation of feline sarcoma-related protein inhibits cell migration, invasion and epithelial-mesenchymal transition via the ERK/AP-1 pathway in bladder urothelial cell carcinoma. Oncol Lett 13:686-694 (2017). WB . PubMed: 28356947
- McGinnis LK et al. Fer tyrosine kinase is required for germinal vesicle breakdown and meiosis-I in mouse oocytes. Mol Reprod Dev 78:33-47 (2011). IHC-P, IF ; Mouse . PubMed: 21268181
- Whitehead SN et al. Transient and bilateral increase in Neuropilin-1, Fer kinase and collapsin response mediator proteins within membrane rafts following unilateral occlusion of the middle cerebral artery in mouse. Brain Res 1344:209-16 (2010). WB ; Mouse . PubMed: 20493826
- Fei F et al. The Fer tyrosine kinase regulates interactions of Rho GDP-Dissociation Inhibitor a with the small GTPase Rac. BMC Biochem 11:48 (2010). WB ; Human, Rat . PubMed: 21122136