概述

  • 产品名称
  • 描述
    兔多克隆抗体to FEN1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Cow, Human
  • 免疫原

    Synthetic peptide derived from within residues 100 - 200 of Human FEN1.

    (Peptide available as ab73361.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Mouse Liver, Mouse Kidney, Rat Kidney

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab73362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能
    Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
  • 序列相似性
    Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
  • 翻译后修饰
    Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
    Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
    Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
  • 细胞定位
    Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNase IV antibody
    • FEN-1 antibody
    • FEN1 antibody
    • FEN1_HUMAN antibody
    • Flap endonuclease 1 antibody
    • Flap structure specific endonuclease 1 antibody
    • Flap structure-specific endonuclease 1 antibody
    • hFEN-1 antibody
    • hFEN1 antibody
    • Maturation factor 1 antibody
    • MF1 antibody
    • Rad2 antibody
    see all

图片

  • All lanes : Anti-FEN1 antibody (ab73362) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Kidney (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab73362 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Nov 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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