Synthetic peptide representing human Flap Structure-specific Endonuclease I encoded within exon 2 (LocusLink ID 2237). The peptide is derived from a portion of FEN1 that begins a few residues downstream from the I-domain as defined in Swiss-Prot entry P39748.
Our Abpromise guarantee covers the use of ab17994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/200. | |
WB | 1/5000 - 1/60000. Detects a band of approximately 47 kDa (predicted molecular weight: 42 kDa). | |
IP | Use at 4-10 µg/mg of lysate. | |
IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ab17994 at the indicated concentrations, staining human FEN1 in (20
ab17994 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"