概述
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产品名称Anti-FEN1抗体
参阅全部 FEN1 一抗 -
描述兔多克隆抗体to FEN1
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宿主Rabbit
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经测试应用适用于: ICC/IF, WB, IP, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Sheep, Horse, Cow, Pig, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Bat
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免疫原
Synthetic peptide representing human Flap Structure-specific Endonuclease I encoded within exon 2 (LocusLink ID 2237). The peptide is derived from a portion of FEN1 that begins a few residues downstream from the I-domain as defined in Swiss-Prot entry P39748.
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阳性对照
- HeLa cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab17994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/200. | |
| WB | 1/5000 - 1/60000. Detects a band of approximately 47 kDa (predicted molecular weight: 42 kDa). | |
| IP | Use at 4-10 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
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序列相似性Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
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翻译后修饰Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. -
细胞定位Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
- Information by UniProt
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数据库链接
- Entrez Gene: 616242 Cow
- Entrez Gene: 2237 Human
- Omim: 600393 Human
- SwissProt: Q58DH8 Cow
- SwissProt: P39748 Human
- Unigene: 409065 Human
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别名
- DNase IV antibody
- FEN-1 antibody
- FEN1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody (ab17994)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FEN1 with ab17994 at 1/500 (2µg/ml). Detection: DAB. -
Western blot - Anti-FEN1 antibody (ab17994)ab17994 at the indicated concentrations, staining human FEN1 in (20
ab17994 at the indicated concentrations, staining human FEN1 in (20µg) HeLa cell lysate by Western blot. Secondary antibody coonjugated to HRP used at 1/40000 (for 0.015 µg/ml anti-Fen1) or 1/60000 (for 1 and 0.1µg/ml anti-Fen1). Detection was by chemiluminescence with the indicated exposure.µ g) HeLa cell lysate by Western blot. Secondary antibody coonjugated to HRP used at 1/40000 (for 0.015µ g/ml anti-Fen1) or 1/60000 (for 1 and 0.1µ g/ml anti-Fen1). Detection was by chemiluminescence with the indicated exposure. -
Immunocytochemistry/ Immunofluorescence - Anti-FEN1 antibody (ab17994)This image is courtesy of an Abreview submitted by Dr Kirk McManusab17994 (1/200) staining FEN1 in HeLa cells. Cells were fixed in Paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.
实验方案
文献
ab17994 has not yet been referenced specifically in any publications.
客户评价及客户问答
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