The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FACS: Use 50µl for 106 cells in 100µl.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
HLA-DRB1 belongs to the HLA class II beta chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells including B lymphocytes, dendritic cells and macrophages.