概述

  • 产品名称
  • 描述
    兔多克隆抗体to FECH
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee, Zebrafish
  • 免疫原

    A region within synthetic peptide: PFIAKRRTPK IQEQYRRIGG GSPIKIWTSK QGEGMVKLLD ELSPNTAPHK, corresponding to N terminal amino acids 109-158 of Human FECH

  • 阳性对照
    • Jurkat mitochondrial lysate

性能

应用

Our Abpromise guarantee covers the use of ab55965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Catalyzes the ferrous insertion into protoporphyrin IX.
  • 通路
    Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.
  • 疾病相关
    Erythropoietic protoporphyria (EPP) [MIM:177000]: A form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. Erythropoietic protoporphyria is marked by excessive protoporphyrin in erythrocytes, plasma, liver and feces, and by widely varying photosensitive skin changes ranging from a burning or pruritic sensation to erythema, edema and wheals. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • 序列相似性
    Belongs to the ferrochelatase family.
  • 细胞定位
    Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EPP antibody
    • FCE antibody
    • Fch antibody
    • Fcl antibody
    • fech antibody
    • Ferrochelatase (protoporphyria) antibody
    • Ferrochelatase antibody
    • Ferrochelatase mitochondrial antibody
    • Heme synthase antibody
    • Heme synthetase antibody
    • HEMH_HUMAN antibody
    • mitochondrial antibody
    • Protoheme ferro lyase antibody
    • Protoheme ferro-lyase antibody
    • Protoporphyria antibody
    see all

图片

  • Anti-FECH antibody (ab55965) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab55965 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Lim SC  et al. Mutations in LYRM4, encoding iron-sulfur cluster biogenesis factor ISD11, cause deficiency of multiple respiratory chain complexes. Hum Mol Genet 22:4460-73 (2013). Read more (PubMed: 23814038) »
  • Gupta V  et al. Salicylic acid induces mitochondrial injury by inhibiting ferrochelatase heme biosynthesis activity. Mol Pharmacol 84:824-33 (2013). Human . Read more (PubMed: 24043703) »

See all 2 Publications for this product

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