概述

  • 产品名称Anti-FE65抗体- ChIP Grade
    参阅全部 FE65 一抗
  • 描述
    兔多克隆抗体to FE65 - ChIP Grade
  • 特异性This antibody does not detect endogenous levels of Fe65.
  • 经测试应用适用于: ICC, WB, ChIPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Mouse FE65 aa 352-369.
    Sequence:

    PQEEEKLSQRNANPGIKC


    (Peptide available as ab5892)

  • 阳性对照
    • Mouse brain lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab5668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
WB Use a concentration of 0.2 µg/ml. Detects a band of approximately 97 kDa.Can be blocked with FE65 peptide (ab5892).
ChIP Use at an assay dependent concentration. PubMed: 1755654

靶标

  • 功能Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).
  • 组织特异性Highly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease.
  • 序列相似性Contains 2 PID domains.
    Contains 1 WW domain.
  • 翻译后修饰Phosphorylated following nuclear translocation. Phosphorylation at Tyr-546 enhances the transcription activation activity and reduces the affinity with RASD1/DEXRAS1.
  • 细胞定位Cell membrane. Cytoplasm. Nucleus. Cell projection > growth cone. Colocalizes with TSHZ3 in axonal growth cone (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adaptor protein FE65a2 antibody
    • Amyloid beta (A4) precursor protein binding family B member 1 antibody
    • Amyloid beta A4 precursor protein binding family B antibody
    • Amyloid beta A4 precursor protein binding family B member 1 antibody
    • Amyloid beta A4 precursor protein-binding family B member 1 antibody
    • Amyloid beta precursor protein binding family B member 1 antibody
    • APBB 1 antibody
    • APBB1 antibody
    • APBB1_HUMAN antibody
    • FE 65 antibody
    • Fe65 protein antibody
    • Protein Fe65 antibody
    • RIR antibody
    • stat like protein antibody
    see all

Anti-FE65 antibody - ChIP Grade 图像

  • Western blot of Fe65-affinity pulldown materials from mouse brain lysate using ab5668. Lane 1 is an immunopurified sample from a full-length Fe65 Knockout mouse. Lane 2 is an immunopurified sample from an Fe65 wild type mouse.

Anti-FE65 antibody - ChIP Grade (ab5668)参考文献

This product has been referenced in:
  • Zhang YW  et al. Presenilin/gamma-secretase-dependent processing of beta-amyloid precursor protein regulates EGF receptor expression. Proc Natl Acad Sci U S A 104:10613-8 (2007). ChIP ; Mouse . Read more (PubMed: 17556541) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with human has not yet been tested. Ab5668 has only been tested for application in Western blotting so...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"