概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to FDFT1
  • 宿主
    Mouse
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Recombinant fragment
    预测可用于: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    EFLDKHVTSEQEWDKYCHYVAGLVGIGLSRLFSASEFEDPLVGEDTERAN SMGLFLQKTNIIRDYLEDQQGGREFWPQEVWSRYVKKLGDFAKPENIDLA

    , corresponding to amino acids 156/255 of Human FDFT1

  • 常规说明
    Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

应用

Our Abpromise guarantee covers the use of ab24429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 48 kDa.

靶标

图片

  • All lanes : Anti-FDFT1 antibody (ab24429) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
    negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
    antigen fusion protein at 20 ug

    Secondary
    All lanes : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
    1/5000 dilution


    Predicted band size: 48 kDa



    The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.

实验方案

文献

This product has been referenced in:
  • Rice LM  et al. Protein Phosphatase 2A (PP2A) Regulates Low Density Lipoprotein Uptake through Regulating Sterol Response Element-binding Protein-2 (SREBP-2) DNA Binding. J Biol Chem 289:17268-17279 (2014). WB . Read more (PubMed: 24770487) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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