概述

  • 产品名称Anti-FCN1抗体
    参阅全部 FCN1 一抗
  • 描述
    小鼠单克隆抗体to FCN1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: SSELRVDLVD FEGNHQFAKY KSFKVADEAE KYKLVLGAFV GGSAGNSLTG HNNNFFSTKD QDNDVSSSNC AEKFQGAWWY ADCHASNLNG LYLMGPHESY, corresponding to amino acids 201-300 of Human FCN1 (NP_001994) with a 26 kDa tag.

  • 阳性对照
    • FCN1 transfected 293T cell lysate; Recombinant tagged human FCN1 fragment.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged FCN1 is approximately 0.3ng/ml when used as a capture antibody.

靶标

  • 功能Involved in serum exerting lectin activity. Binds GlcNAc.
  • 组织特异性Peripheral blood leukocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes and granulocytes.
  • 序列相似性Belongs to the ficolin lectin family.
    Contains 1 collagen-like domain.
    Contains 1 fibrinogen C-terminal domain.
  • 细胞定位Secreted. Found on the monocyte surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Collagen/fibrinogen domain containing protein 1 antibody
    • Collagen/fibrinogen domain-containing protein 1 antibody
    • Fcn1 antibody
    • FCN1_HUMAN antibody
    • FCNM antibody
    • ficolin (collagen/fibrinogen domain containing) 1 antibody
    • Ficolin 1 antibody
    • Ficolin A antibody
    • Ficolin alpha antibody
    • Ficolin-1 antibody
    • Ficolin-A antibody
    • Ficolin-alpha antibody
    • M ficolin antibody
    • M-ficolin antibody
    see all

Anti-FCN1 antibody 图像

  • Anti-FCN1 antibody (ab77454) at 1 µg/ml + Recombinant tagged human FCN1 fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 35 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-FCN1 antibody (ab77454) at 1 µg/ml

    Lane 1 : FCN1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-FCN1 antibody (ab77454)参考文献

ab77454 has not yet been referenced specifically in any publications.

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