Mouse, Rat, Human
antigen sequence: LENSSKVVRD YNLQLFLQEN AVFHKPQPFQ FQPCDSDTSR QLESETGTTE EHSLNKEARK WATRVAREHK NIVHQQRVLN DLECHGAAVS EQSRA, corresponding to amino acids of Human FCHSD2.
Human pancreas tissue; RT 4, U 251 MG, Human liver and tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/250 - 1/500.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Liver, brain, heart, placenta, skeletal muscle, pancreas, lung and kidney.
Contains 1 FCH domain.
Contains 2 SH3 domains.
Information by UniProt
FCH and double SH3 domains 2 antibody
FCH and double SH3 domains protein 2 antibody
Immunocytochemistry/ Immunofluorescence - Anti-FCHSD2 antibody (ab122485)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus, plasma membrane and cytoskeleton (actin filaments). Recommended concentration of ab122485 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-FCHSD2 antibody (ab122485)
All lanes : Anti-FCHSD2 antibody (ab122485) at 1/250 dilution Lane 1 : RT 4 cell line lysate Lane 2 : U 251 MG cell line lysate Lane 3 : Human plasma lysate Lane 4 : Human liver tissue lysate Lane 5 : Human tonsil tissue lysate Developed using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCHSD2 antibody (ab122485)
ab122485, at 1/50, staining FCHSD2 in paraffin embedded Human pancreas tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"