概述

  • 产品名称Anti-FCHSD1抗体
  • 描述
    小鼠多克隆抗体to FCHSD1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length human FCHSD1 protein (Isoform 3; AAH47016.1, 1 a.a. ~ 409 a.a)

  • 阳性对照
    • FCHSD1 transfected 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: Isoform 3 46 kDa: Isoform 1 77kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-FCHSD1 antibody 图像

    • All lanes : Anti-FCHSD1 antibody (ab67017) at 1/500 dilution

      Lane 1 : FCHSD1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 46 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Anti-FCHSD1 antibody (ab67017)参考文献

    This product has been referenced in:
    • Cao H  et al. FCHSD1 and FCHSD2 are expressed in hair cell stereocilia and cuticular plate and regulate actin polymerization in vitro. PLoS One 8:e56516 (2013). Mouse . Read more (PubMed: 23437151) »

    See 1 Publication for this product

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

    Read More

    DISCOUNT CODE: 100%ABR-T7AAC
    Expiration date: 7 Aug 2012

    I am very pleased to hear you would like to accept our offer and test ab123596 inrat. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this...

    Read More

    Thank you for contacting us.



    I have received a response from the lab regarding Ab117460 Anti-FCHSD1 antibody. The immunogen sequence is identical with that of rat, but we did not obtain a positive signal in rat tissues, so it is ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (neuro2a cells)
    Loading amount 20 µg
    Specification neuro2a cells
    Treatment FCHSD1 shRNA
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 15 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"