概述

  • 产品名称Anti-FCHO1抗体
    参阅全部 FCHO1 一抗
  • 描述
    兔多克隆抗体to FCHO1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 468-517 (SPENVEDSGL DSPSHAAPGP SPDSWVPRPG TPQSPPSCRA PPPEARGIRA) of Human FCHO1 (NP_055937).

  • 阳性对照
    • 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-FCHO1 antibody 图像

  • Anti-FCHO1 antibody (ab84740) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
  • ab84740 staining FCHO1 in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84740 staining FCHO1 in human uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-FCHO1 antibody (ab84740)参考文献

This product has been referenced in:
  • Umasankar PK  et al. A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing. Elife 3:N/A (2014). Read more (PubMed: 25303365) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"