概述

  • 产品名称Anti-FCHO1抗体
    参阅全部 FCHO1 一抗
  • 描述
    兔多克隆抗体to FCHO1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 93-242 of Human FCHO1 (AAH28021).

  • 阳性对照
    • 293T and Raji cell lysates This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PANC-1.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab102994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 97 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-FCHO1 antibody 图像

  • ICC/IF image of ab102994 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102994 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FCHO1 antibody (ab102994) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 97 kDa
    7.5% SDS PAGE

Anti-FCHO1 antibody (ab102994)参考文献

ab102994 has not yet been referenced specifically in any publications.

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