重组Anti-CD64抗体[EPR4623] (ab109449)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4623] to CD64
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD64抗体[EPR4623]
参阅全部 CD64 一抗 -
描述
兔单克隆抗体[EPR4623] to CD64 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cytmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- U937, THP1, and HL60 cell lysates; U937 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.00 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4623 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109449于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa).
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Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa). |
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. -
组织特异性
Monocyte/macrophage specific. -
序列相似性
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Phosphorylated on serine residues. -
细胞定位
Cell membrane. Stabilized at the cell membrane through interaction with FCER1G. - Information by UniProt
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数据库链接
- Entrez Gene: 2209 Human
- Entrez Gene: 2210 Human
- Omim: 146760 Human
- Omim: 601502 Human
- Omim: 601503 Human
- SwissProt: A6NKC4 Human
- SwissProt: P12314 Human
- SwissProt: Q92637 Human
see all -
别名
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
see all
图片
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All lanes : Anti-CD64 antibody [EPR4623] (ab109449) at 1/1000 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : FCGR1A knockout THP-1 cell lysate
Lane 3 : U937 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD64 antibody [EPR4623] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109449 was shown to bind specifically to CD64. A band was observed at 45-50/55-80 kDa in wild-type THP-1 cell lysates with no signal observed at this size in FCGR1A knockout cell line ab275843 (knockout cell lysate ab275817). To generate this image, wild-type and FCGR1A knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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ab109449 at 1/100 dilution staining CD64 in U937 cells by Flow cytometry (shown in red). Rabbit IgG negative control (shown in green).
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All lanes : Anti-CD64 antibody [EPR4623] (ab109449) at 1/1000 dilution
Lane 1 : U937 cell lysate
Lane 2 : THP1 cell lysate
Lane 3 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Observed band size: 64-78 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab109449 被引用在 2 文献中.
- Zhang H et al. Fc?RI (CD64) contributes to the severity of immune inflammation through regulating NF-?B/NLRP3 inflammasome pathway. Life Sci 207:296-303 (2018). PubMed: 29920250
- Lin HT et al. An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils. Mol Ther Methods Clin Dev 2:15046 (2015). PubMed: 26682238