概述

  • 产品名称Anti-FCER1G抗体
    参阅全部 FCER1G 一抗
  • 描述
    兔多克隆抗体to FCER1G
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MIPAVVLLLL LLVEQAAALG EPQLCYILDA ILFLYGIVLT LLYCRLKIQV) of Human FCER1G (NP_004097).

  • 阳性对照
    • HeLa cell lysate IHC-P (FFPE): Human Lung (Normal)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab108228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Associates with a variety of FcR alpha chains to form a functional signaling complex. Regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface.
  • 序列相似性Belongs to the CD3Z/FCER1G family.
    Contains 1 ITAM domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide antibody
    • Fc receptor gamma chain antibody
    • Fc-epsilon RI-gamma antibody
    • FCER1G antibody
    • FCERG_HUMAN antibody
    • FceRI gamma antibody
    • FCRG antibody
    • FcRgamma antibody
    • High affinity immunoglobulin epsilon receptor subunit gamma antibody
    • IgE Fc receptor subunit gamma antibody
    • Immunoglobulin E receptor, high affinity, gamma chain antibody
    see all

Anti-FCER1G antibody 图像

  • IHC image of FCER1G staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108228, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Anti-FCER1G antibody (ab108228) at 1 µg/ml + HeLa cell lysate at 10 µg

    Predicted band size : 10 kDa
    10-20% gel

Anti-FCER1G antibody (ab108228)参考文献

ab108228 has not yet been referenced specifically in any publications.

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