Anti-Fc epsilon RI抗体[CRA1] (Biotin) (ab122882)

概述

  • 产品名称Anti-Fc epsilon RI抗体[CRA1] (Biotin)
    参阅全部 Fc epsilon RI 一抗
  • 描述
    小鼠单克隆抗体[CRA1] to Fc epsilon RI (Biotin)
  • 偶联物Biotin
  • 特异性ab122882 reacts with the Fc epsilon RI subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete with IgE for the receptor binding. Since clone CRA2 (ab122891; ab122915) reacts with the IgE binding site on Fc epsilon RI, it competes with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound Fc epsilon RI.
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment:

    MAPAMESPTL LCVALLFFAP DGVLAVPQKP KVSLNPPWNR IFKGENVTLT CNGNNFFEVS STKWFHNGSL SEETNSSLNI VNAK

    , corresponding to amino acids 1-84 of Human Fc epsilon RI

  • 表位corresponds to Amino acids 1-84 of Human Fc epsilon RI
  • 阳性对照
    • CHO/alpha beta gamma cells
  • 常规说明

    [biotin]/[IgG] = 8.9

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明The IgG fraction was purified from serum free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions. Filter sterilized.
  • 克隆单克隆
  • 克隆编号CRA1
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
Flow Cyt Use at an assay dependent concentration. ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
  • 序列相似性Contains 2 Ig-like (immunoglobulin-like) domains.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Fc epsilon RI alpha antibody
    • Fc epsilon RI alpha chain antibody
    • Fc epsilon RI alpha-chain antibody
    • Fc fragment of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc fragment of IgE, high affinity I, receptor for, alpha subunit antibody
    • Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide antibody
    • Fc IgE receptor alpha polypeptide antibody
    • Fc IgE receptor, alpha chain antibody
    • Fc IgE receptor, alpha polypeptide antibody
    • Fc of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc-epsilon RI-alpha antibody
    • FCE 1A antibody
    • FCE1A antibody
    • FCER 1A antibody
    • Fcer1a antibody
    • FCERA_HUMAN antibody
    • FceRI alpha antibody
    • FcERI antibody
    • high affinity IgE receptor antibody
    • High affinity immunoglobulin epsilon receptor alpha subunit antibody
    • high affinity immunoglobulin epsilon receptor alpha-subunit antibody
    • High affinity immunoglobulin epsilon receptor subunit alpha antibody
    • IgE Fc receptor alpha subunit antibody
    • IgE Fc receptor subunit alpha antibody
    • Immunoglobulin E receptor high affinity of mast cells alpha polypeptide antibody
    • immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody
    see all

Anti-Fc epsilon RI antibody [CRA1] (Biotin) 图像

  • FACS analysis of CHO/alpha beta gamma cells (1x105) with CRA1 and CRA2 antibodies by indirect-immunostaining, using FITC-labeled secondary antibody.

Anti-Fc epsilon RI antibody [CRA1] (Biotin) (ab122882)参考文献

This product has been referenced in:
  • Untersmayr E  et al. The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. PLoS One 5:e9023 (2010). Read more (PubMed: 20126404) »
  • Takai T  et al. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. Biosci Biotechnol Biochem 65:79-85 (2001). Read more (PubMed: 11272849) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab122882.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"