• 产品名称Anti-Fbxw7抗体
    参阅全部 Fbxw7 一抗
  • 描述
    兔多克隆抗体to Fbxw7
  • 经测试应用适用于: IHC-P, WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Chicken, Cow, Pig, Chimpanzee, Zebrafish, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Fbxw7.

  • 阳性对照
    • WB: HepG2, HCT116, PANC-1 and MCF7 cell lysates. IHC-P Human normal colon tissue. ICC/IF: HepG2 cells.



Our Abpromise guarantee covers the use of ab109617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 79 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.


  • 功能Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
  • 组织特异性Isoform 1 is widely expressed. Isoform 4 is expressed in brain.
  • 序列相似性Contains 1 F-box domain.
    Contains 7 WD repeats.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm; Nucleus; Nucleus > nucleolus and Nucleus > nucleoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
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    • F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) antibody
    • F box and WD 40 domain protein 7 antibody
    • F box and WD repeat domain containing 7 antibody
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    • F box protein FBX30 antibody
    • F box protein SEL 10 antibody
    • F box protein SEL10 antibody
    • F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase antibody
    • F-box and WD-40 domain-containing protein 7 antibody
    • F-box protein FBX30 antibody
    • F-box/WD repeat-containing protein 7 antibody
    • FBW6 antibody
    • FBW7 antibody
    • FBX30 antibody
    • FBXO30 antibody
    • FBXW6 antibody
    • FBXW7 antibody
    • FBXW7_HUMAN antibody
    • FLJ16457 antibody
    • hAgo antibody
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    • Homolog of C elegans sel 10 antibody
    • Homolog of C.elegans sel10 antibody
    • SEL 10 antibody
    • SEL-10 antibody
    • SEL10 antibody
    see all

Anti-Fbxw7 antibody 图像

  • Fbxw7 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to Fbxw7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab109617.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 69kDa; Fbxw7

  • ICC/IF image of ab109617 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109617, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Fbxw7 antibody (ab109617) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
    Lane 3 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 79 kDa
    Observed band size : 69 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
    The predicted molecular weight of Fbxw7 is 79 kDa (SwissProt), however we expect to observe a banding pattern at 69 kDa within Human lysates. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109617 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • IHC image of Fbxw7 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109617, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Fbxw7 antibody (ab109617)参考文献

ab109617 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Primary bone marrow-derived macrophages)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification Primary bone marrow-derived macrophages
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Jeff Deiuliis

Verified customer

提交于 Apr 11 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% TGX BioRad precast gels)
Sample Monkey Cell lysate - whole cell (kidney)
Specification kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Oct 30 2014