Anti-FBXO7抗体(ab84129)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to FBXO7
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the middle amino acids 467-516 (

    PGETPSQFPPLRPRFDPVGPLPGPNPILPGRGGPNDRFPFRPSRGRPTDG

    ) of Human FBXO7 (NP_036311)

  • 阳性对照
    • MCF7 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab84129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes BIRC2 and DLGAP5.
  • 通路
    Protein modification; protein ubiquitination.
  • 疾病相关
    Defects in FBXO7 are the cause of Parkinson disease type 15 (PARK15) [MIM:260300]; also known as parkinsonian-pyramidal syndrome. A neurodegenerative disorder characterized by parkinsonian and pyramidal signs. Clinical manifestations include tremor, bradykinesia, rigidity, postural instability, spasticity, mainly in the lower limbs, and hyperreflexia.
  • 序列相似性
    Contains 1 F-box domain.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • F box only protein 7 antibody
    • F box protein 7 antibody
    • F-box only protein 7 antibody
    • FBX antibody
    • FBX07 antibody
    • FBX7 antibody
    • FBX7_HUMAN antibody
    • Fbxo7 antibody
    • PARK15 antibody
    • PKPS antibody
    see all

图片

  • Western blot analysis of HeLa cell lysate labeling FBXO7 with ab84129 at 1.0µg/ml.
  • Western blot analysis of 293T cell lysate labeling FBXO7 with ab84129 at 1.0µg/ml.
  • Anti-FBXO7 antibody (ab84129) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59,70 kDa (why is the actual band size different from the predicted?)

文献

ab84129 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 24 2018

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Human Cell (HCC 1954 - Breast cancer cell lines)
Specification
HCC 1954 - Breast cancer cell lines
Permeabilization
Yes - 0.3% Triton X-100 in TBS
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 31 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (Daoy)
Specification
Daoy
Treatment
20 nM siRNA for 72 hours
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Nov 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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