概述

  • 产品名称
    Anti-FBXO7抗体
    参阅全部 FBXO7 一抗
  • 描述
    兔多克隆抗体to FBXO7
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the middle amino acids 467-516 (

    PGETPSQFPPLRPRFDPVGPLPGPNPILPGRGGPNDRFPFRPSRGRPTDG

    ) of Human FBXO7 (NP_036311)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • MCF7 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab84129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes BIRC2 and DLGAP5.
  • 通路
    Protein modification; protein ubiquitination.
  • 疾病相关
    Defects in FBXO7 are the cause of Parkinson disease type 15 (PARK15) [MIM:260300]; also known as parkinsonian-pyramidal syndrome. A neurodegenerative disorder characterized by parkinsonian and pyramidal signs. Clinical manifestations include tremor, bradykinesia, rigidity, postural instability, spasticity, mainly in the lower limbs, and hyperreflexia.
  • 序列相似性
    Contains 1 F-box domain.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • F box only protein 7 antibody
    • F box protein 7 antibody
    • F-box only protein 7 antibody
    • FBX antibody
    • FBX07 antibody
    • FBX7 antibody
    • FBX7_HUMAN antibody
    • Fbxo7 antibody
    • PARK15 antibody
    • PKPS antibody
    see all

图片

  • Western blot - Anti-FBXO7 antibody (ab84129)
    Western blot - Anti-FBXO7 antibody (ab84129)
    Western blot analysis of HeLa cell lysate labeling FBXO7 with ab84129 at 1.0µg/ml.
  • Western blot - Anti-FBXO7 antibody (ab84129)
    Western blot - Anti-FBXO7 antibody (ab84129)
    Western blot analysis of 293T cell lysate labeling FBXO7 with ab84129 at 1.0µg/ml.
  • Western blot - Anti-FBXO7 antibody (ab84129)
    Western blot - Anti-FBXO7 antibody (ab84129)
    Anti-FBXO7 antibody (ab84129) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59,70 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Song T  et al. CRL4 antagonizes SCFFbxo7-mediated turnover of cereblon and BK channel to regulate learning and memory. PLoS Genet 14:e1007165 (2018). Read more (PubMed: 29370161) »

See 1 Publication for this product

发表研究结果有使用 ab84129？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Western blot abreview for Anti-FBXO7 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Jan 24 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-FBXO7 antibody

 Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Human Cell (HCC 1954 - Breast cancer cell lines)
Specification
HCC 1954 - Breast cancer cell lines
Permeabilization
Yes - 0.3% Triton X-100 in TBS
Fixative
Formaldehyde
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Verified customer

提交于 Dec 31 2014

Western blot abreview for Anti-FBXO7 antibody

 Good
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (Daoy)
Specification
Daoy
Treatment
20 nM siRNA for 72 hours
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
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提交于 Nov 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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