概述

  • 产品名称Anti-FBXO4抗体
    参阅全部 FBXO4 一抗
  • 描述
    兔多克隆抗体to FBXO4
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (TSAVNKMFSR HNEGDDQQGS RYSVIPQIQK VCEVVDGFIY VANAEAHKSK) of Human FBXO4 (NP_277019).

  • 阳性对照
    • 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 功能Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 F-box domain.
  • 翻译后修饰Phosphorylation at Ser-12 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase (By similarity). Phosphorylation at Ser-12 promotes homodimerization and is necessary for optimal ubiquitin ligase activity towards CCND1.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp547N213 antibody
    • F box only protein 4 antibody
    • F box protein 4 antibody
    • F box protein Fbx4 antibody
    • F-box only protein 4 antibody
    • FBX4 antibody
    • FBX4_HUMAN antibody
    • FBXO 4 antibody
    • FBXO4 antibody
    • FLJ10141 antibody
    see all

Anti-FBXO4 antibody 图像

  • Anti-FBXO4 antibody (ab83318) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

Anti-FBXO4 antibody (ab83318)参考文献

ab83318 has not yet been referenced specifically in any publications.

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