概述

  • 产品名称
    Anti-FBXO25抗体
  • 描述
    兔多克隆抗体to FBXO25
  • 特异性
    ab82971 detects isoform 3.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (LGEAFNRLDF SSAIQDIRRF NYVVKLLQLI AKSQLTSLSG VAQKNYFNIL) of human FBXO25 (NP_036305).

  • 阳性对照
    • Fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab82971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1/312500.

靶标

  • 功能
    Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT).
  • 组织特异性
    Expressed in all brain tissue observed.
  • 通路
    Protein modification; protein ubiquitination.
  • 疾病相关
    Note=A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
  • 序列相似性
    Contains 1 F-box domain.
  • 结构域
    The F-box is necessary for the interaction with SKP1.
  • 细胞定位
    Nucleus. In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • F box only protein 25 antibody
    • F box protein 25 antibody
    • F box protein Fbx25 antibody
    • F-box only protein 25 antibody
    • FBX25 antibody
    • FBX25_HUMAN antibody
    • FBXO25 antibody
    • MGC20256 antibody
    • MGC51975 antibody
    • OTTHUMP00000115399 antibody
    see all

图片



  • Predicted band size : 34 kDa
    Western bloat analysis of human brain tissue lysate labelling FBXO25 with ab82971 at 0.2-1µg/ml.
  • Anti-FBXO25 antibody (ab82971) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

实验方案

文献

ab82971 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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