ab82971 detects isoform 3.
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (LGEAFNRLDF SSAIQDIRRF NYVVKLLQLI AKSQLTSLSG VAQKNYFNIL) of human FBXO25 (NP_036305).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
ab82971 is purified by a peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1/312500.
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT).
Expressed in all brain tissue observed.
Protein modification; protein ubiquitination.
Note=A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
Contains 1 F-box domain.
The F-box is necessary for the interaction with SKP1.
Nucleus. In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1.
Information by UniProt
- F box only protein 25 antibody
- F box protein 25 antibody
- F box protein Fbx25 antibody
Western blot - Anti-FBXO25 antibody (ab82971)
Western bloat analysis of human brain tissue lysate labelling FBXO25 with ab82971 at 0.2-1µg/ml.
Western blot - FBXO25 antibody (ab82971)
Anti-FBXO25 antibody (ab82971) at 1 µg/ml + fetal heart lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
has not yet been referenced specifically in any publications.
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