Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
A region within synthetic peptide: EQYGDTLHFC RHCSILFWKD SGHPCTAADP DSCFTPVSPQ HFIDLFKF, corresponding to C terminal amino acids 244-291 of Human FBXO25
HepG2 cell lysate; human kidney tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
Use a concentration of 1.25 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT).
Expressed in all brain tissue observed.
Protein modification; protein ubiquitination.
Note=A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
Contains 1 F-box domain.
The F-box is necessary for the interaction with SKP1.
Nucleus. In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1.
Information by UniProt
F box only protein 25 antibody
F box protein 25 antibody
F box protein Fbx25 antibody
Anti-FBXO25 antibody 图像
Western blot - FBXO25 antibody (ab58784)
Anti-FBXO25 antibody (ab58784) at 1.25 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS buffer
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size :
Observed band size :
20 kDa (
why is the actual band size different from the predicted?
Gel concentration 12%
Immunohistochemistry (Paraffin-embedded sections) - FBXO25 antibody (ab58784)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FBXO25 with ab58784 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab58784 at a dilution of 4-8 µg/ml, positively labelling epithelial cells of renal tubule (indicated with arrows). Magnification 400X
Anti-FBXO25 antibody (ab58784)参考文献
has not yet been referenced specifically in any publications.
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