Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
Synthetic peptide corresponding to a region within internal sequence amino acids 227-276 (CCKVGASNYL QQVVSTFSDM NIILAGGQVD NLSSLTSEKY VLCASDFVCE) of Human FBXO22 (NP_036302).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/12500.
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Predominantly expressed in liver.
Contains 1 F-box domain.
Information by UniProt
- 0610033L19Rik antibody
- 1600016C16Rik antibody
- F box only protein 22 antibody
Western blot - FBXO22 antibody (ab82974)
Anti-FBXO22 antibody (ab82974) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
30 kDaObserved band size:
28 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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