Anti-FbxL7抗体(ab59149)

概述

  • 产品名称
    Anti-FbxL7抗体
  • 描述
    兔多克隆抗体to FbxL7
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • 免疫原

    Synthetic peptide: KEQASIDRLP DHSMVQIFSF LPTNQLCRCA RVCRRWYNLA WDPRLWRTIR, corresponding to amino acids 109-158 of Human FbxL7

  • 阳性对照
    • HepG2 cell lysate and human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab59149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay:1/312500.

靶标

  • 功能
    Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
  • 序列相似性
    Contains 1 F-box domain.
    Contains 11 LRR (leucine-rich) repeats.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AL023057 antibody
    • D230018M15Rik antibody
    • F Box and Leucine Rich Repeat Protein 7 antibody
    • F box protein FBL6/FBL7 antibody
    • F box/LRR-repeat protein 7 antibody
    • F-box and leucine-rich repeat protein 7 antibody
    • F-box protein FBL6/FBL7 antibody
    • F-box/LRR-repeat protein 7 antibody
    • FBL 6 antibody
    • FBL 7 antibody
    • FBL6 antibody
    • FBL7 antibody
    • FBXL 7 antibody
    • FBXL7 antibody
    • FBXL7_HUMAN antibody
    • FLJ11609 antibody
    • KIAA0840 antibody
    • MGC102204 antibody
    see all

图片

  • Anti-FbxL7 antibody (ab59149) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FbxL7 with ab59149 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

文献

ab59149 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Jan 04 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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