概述

  • 产品名称Anti-FbxL7抗体
  • 描述
    兔多克隆抗体to FbxL7
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • 免疫原

    Synthetic peptide: KEQASIDRLP DHSMVQIFSF LPTNQLCRCA RVCRRWYNLA WDPRLWRTIR, corresponding to amino acids 109-158 of Human FbxL7

  • 阳性对照
    • HepG2 cell lysate and human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab59149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay:1/312500.

靶标

  • 功能Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
  • 序列相似性Contains 1 F-box domain.
    Contains 11 LRR (leucine-rich) repeats.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AL023057 antibody
    • D230018M15Rik antibody
    • F Box and Leucine Rich Repeat Protein 7 antibody
    • F box protein FBL6/FBL7 antibody
    • F box/LRR-repeat protein 7 antibody
    • F-box and leucine-rich repeat protein 7 antibody
    • F-box protein FBL6/FBL7 antibody
    • F-box/LRR-repeat protein 7 antibody
    • FBL 6 antibody
    • FBL 7 antibody
    • FBL6 antibody
    • FBL7 antibody
    • FBXL 7 antibody
    • FBXL7 antibody
    • FBXL7_HUMAN antibody
    • FLJ11609 antibody
    • KIAA0840 antibody
    • MGC102204 antibody
    see all

Anti-FbxL7 antibody 图像

  • Anti-FbxL7 antibody (ab59149) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FbxL7 with ab59149 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-FbxL7 antibody (ab59149)参考文献

ab59149 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab59149.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"