Anti-FBXL3抗体(ab115515)
Key features and details
- Goat polyclonal to FBXL3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FBXL3抗体
参阅全部 FBXL3 一抗 -
描述
山羊多克隆抗体to FBXL3 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Dog -
免疫原
Synthetic peptide:
KRGGRDSDRNSSEE-C
, corresponding to N terminal amino acids 2-15 of Human FBXL3. -
阳性对照
- Human thyroid tissue; Human adipose lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115515于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 49 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 49 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex involved in circadian clock function. The SCF(FBXL3) complex acts by mediating ubiquitination and subsequent degradation of CRY1 and CRY2. Recruiter of target protein that may recognize and bind to some phosphorylated proteins and promotes their ubiquitination and degradation. -
组织特异性
Widely expressed. -
序列相似性
Contains 1 F-box domain.
Contains 7 LRR (leucine-rich) repeats. -
细胞定位
Nucleus. Cytoplasm. Predominantly nuclear. - Information by UniProt
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数据库链接
- Entrez Gene: 485499 Dog
- Entrez Gene: 26224 Human
- Omim: 605653 Human
- SwissProt: Q9UKT7 Human
- Unigene: 508284 Human
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别名
- F box and leucine rich repeat protein 3 antibody
- F box and leucine rich repeat protein 3A antibody
- F box protein Fbl3a antibody
see all
图片
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ab115515, at 5µg/ml staining FBXL3 in formalin-fixed, paraffin-embedded Human thyroid tissue by Immunohistochemistry, using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Anti-FBXL3 antibody (ab115515) at 1 µg/ml + Human adipose lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Primary incubation: 1 hour
数据表及文件
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Datasheet download
文献 (0)
ab115515 尚未被引用在任何文献中。