The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human FBXL2 peptide (ab23301).
Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin (By similarity). Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.
Expressed in brain, heart, kidney, liver, lung, pancreas and placenta.
ab17018 (10µg/ml) staining of FBXL2 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoplasm staining in neuronal bodies.
Western blot - FBXL2 antibody (ab17018)
ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence. ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence.