Overlay histogram showing A431 cells stained with ab57768 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57768, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
IHC-P - FBXL18 antibody (ab57768)
FBXL18 antibody (ab57768) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.
Western blot - FBXL18 antibody (ab57768)
Predicted band size : 88 kDa FBXL18 antibody (ab57768) at 1ug/lane + A-431 cell lysate at 25ug/lane.
Anti-FBXL18 antibody (ab57768)参考文献
has not yet been referenced specifically in any publications.