概述

  • 产品名称Anti-FBXL15抗体
    参阅全部 FBXL15 一抗
  • 描述
    兔多克隆抗体to FBXL15
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 260-290 of Human FBXL15.

  • 阳性对照
    • MDA-MB435 cell line lysates. Mouse kidney tissue lysates. Human breast carcinoma. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HCT116

性能

应用

Our Abpromise guarantee covers the use of ab103719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 33 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性FBXL15 probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
  • 数据库链接
  • 别名
    • F box and leucine rich repeat protein 15 antibody
    • F box only protein 37 antibody
    • F box/LRR repeat protein 15 antibody
    • Fbl15 antibody
    • FBXO37 antibody
    • FLJ16137 antibody
    • JET antibody
    • MGC11279 antibody
    • OTTHUMP00000020367 antibody
    • OTTHUMP00000059115 antibody
    • OTTHUMP00000059124 antibody
    • OTTHUMP00000059125 antibody
    • Pleckstrin and Sec7 domain protein antibody
    • PSD antibody
    see all

Anti-FBXL15 antibody 图像

  • ab103719 stained HCT116 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103719 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-FBXL15 antibody (ab103719) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 33 kDa
  • Anti-FBXL15 antibody (ab103719) at 1/100 dilution + Mouse kidney tissue lysates at 35 µg

    Predicted band size : 33 kDa
  • ab103719 at 1/50 dilution staining FBXL15 by immunohistochemistry in formalin fixed and paraffin embedded Human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-FBXL15 antibody (ab103719)参考文献

ab103719 has not yet been referenced specifically in any publications.

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