Anti-Fbx32抗体(ab92281)
Key features and details
- Goat polyclonal to Fbx32
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Fbx32抗体
参阅全部 Fbx32 一抗 -
描述
山羊多克隆抗体to Fbx32 -
宿主
Goat -
特异性
Expected to recognize reported isoform 1 (NP_478136.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Cow, Human, Pig -
免疫原
Synthetic peptide:
C-NSKTKTQYFHQEK
, corresponding to internal amino acids 70-82 of Human Fbx32 (NP_478136.1).(Peptide available as ab131394.) -
阳性对照
- WB: Mouse skeletal muscle lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92281于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. -
组织特异性
Specifically expressed in cardiac and skeletal muscle. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 F-box domain. - Information by UniProt
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数据库链接
- Entrez Gene: 513776 Cow
- Entrez Gene: 114907 Human
- Entrez Gene: 67731 Mouse
- Entrez Gene: 733657 Pig
- Entrez Gene: 171043 Rat
- Omim: 606604 Human
- SwissProt: Q2KHT6 Cow
- SwissProt: Q969P5 Human
see all -
别名
- 4833442G10Rik antibody
- AI430017 antibody
- Atrogin 1 antibody
see all
图片
数据表及文件
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Datasheet download
文献 (10)
ab92281 被引用在 10 文献中.
- Järve A et al. Angiotensin-(1-7) Receptor Mas Deficiency Does Not Exacerbate Cardiac Atrophy Following High-Level Spinal Cord Injury in Mice. Front Physiol 11:203 (2020). PubMed: 32226394
- Mose M et al. A model mimicking catabolic inflammatory disease; a controlled randomized study in humans. PLoS One 15:e0241274 (2020). PubMed: 33151986
- Møller AB et al. Molecular and cellular adaptations to exercise training in skeletal muscle from cancer patients treated with chemotherapy. J Cancer Res Clin Oncol 145:1449-1460 (2019). PubMed: 30968255
- Dalgaard LB et al. Influence of Oral Contraceptive Use on Adaptations to Resistance Training. Front Physiol 10:824 (2019). PubMed: 31312144
- Møller AB et al. Immobilization Decreases FOXO3a Phosphorylation and Increases Autophagy-Related Gene and Protein Expression in Human Skeletal Muscle. Front Physiol 10:736 (2019). PubMed: 31258486
- Majumder A et al. Hydrogen sulfide alleviates hyperhomocysteinemia-mediated skeletal muscle atrophy via mitigation of oxidative and endoplasmic reticulum stress injury. Am J Physiol Cell Physiol 315:C609-C622 (2018). PubMed: 30110564
- Wilkinson DJ et al. Impact of the calcium form of ß-hydroxy-ß-methylbutyrate upon human skeletal muscle protein metabolism. Clin Nutr N/A:N/A (2017). PubMed: 29097038
- Apró W et al. Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling. Am J Physiol Endocrinol Metab 308:E470-81 (2015). PubMed: 25605643
- Vendelbo MH et al. Fasting increases human skeletal muscle net phenylalanine release and this is associated with decreased mTOR signaling. PLoS One 9:e102031 (2014). WB ; Human . PubMed: 25020061
- Borgenvik M et al. Intake of branched-chain amino acids influences the levels of MAFbx mRNA and MuRF-1 total protein in resting and exercising human muscle. Am J Physiol Endocrinol Metab 302:E510-21 (2012). WB ; Human . PubMed: 22127230