- 数据表
- 具体的参考文献 (7)
- 实验方案
概述
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产品名称Anti-Fbx32抗体
参阅全部 Fbx32 一抗 -
描述兔多克隆抗体to Fbx32
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宿主Rabbit
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特异性This antibody is only batch tested in WB and therefore we can only guarantee it for this application. Customers have successfully used this antibody in IHC and ICC but we have not tested it's performance in these applications. If you would like an antibody that is tested in ICC, we would recommend our recombinant antibody ab168372.
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经测试应用适用于: WBmore details
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种属反应性与反应: Mouse, Rat, Human, Pig
预测可用于: Chicken, Cow, Zebrafish
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免疫原
Synthetic peptide within Human Fbx32 aa 300 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab74022) -
阳性对照
- WB: Human, mouse and rat skeletal muscle tissue lysate. IHC-P: Human heart tissue. IHC-Fr: Rat muscle tissue. ICC/IF: Rat astrocyte cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab74023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
靶标
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功能Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
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组织特异性Specifically expressed in cardiac and skeletal muscle.
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通路Protein modification; protein ubiquitination.
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序列相似性Contains 1 F-box domain.
- Information by UniProt
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数据库链接
- Entrez Gene: 513776 Cow
- Entrez Gene: 114907 Human
- Entrez Gene: 67731 Mouse
- Entrez Gene: 733657 Pig
- Entrez Gene: 171043 Rat
- Omim: 606604 Human
- SwissProt: Q2KHT6 Cow
- SwissProt: Q969P5 Human
see all -
别名
- 4833442G10Rik antibody
- AI430017 antibody
- Atrogin 1 antibody
see all
图片
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Western blot - Anti-Fbx32 antibody (ab74023)All lanes : Anti-Fbx32 antibody (ab74023) at 2 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Rat skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbx32 antibody (ab74023)This image is courtesy of an abreview submitted by Antibody Solutions Ltd.Paraffin-embedded human heart tissue stained for Fbx32 with ab74023 (1/2,000 dilution) in immunohistochemical analysis.
Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti-rabbit conjugated to HRP. For further experimental details please refer to abreview.
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Immunohistochemistry (Frozen sections) - Anti-Fbx32 antibody (ab74023)This image is courtesy of an Abreview submitted by Ruma Raha-ChowdhuryFrozen sectioned rat muscle tissue stained for Fbx32 with ab74023 (1/200 dilution) (green) in immunohistochemical analysis.
Tissue was fixed with 4% PFA overnight. Samples were then blocked with 10% donkey serum for 1 hour at 24°C followed by incubation with ab74023 for 24 hours at 4°C. A pig anti-rabbit Alexa Fluor488® conjugate was used as secondary antibody at a 1/1,000 dilution. Nuclei were stained using the standard Hoesch method and are depicted in blue.
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Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody (ab74023)This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhuryab74023 staining Fbx32 in cultured rat astrocytes by ICC/IF at a 1/500 dilution (green).
The cultured astrocytes were fixed with paraformaldehyde and blocked with 10% donkey serum for 30 minutes at 24°C. Cells were then stained with ab74023 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4.5 hours at 24°C. An Alexa Fluor-488® donkey anti-rabbit polyclonal antibody at 1/1,000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Fbx32 expressed in the newly born astrocytes but does not co-localise with mature astrocytes.
实验方案
文献
This product has been referenced in:
- Zhu X et al. The role of calpains in ventilator-induced diaphragm atrophy. Intensive Care Med Exp 5:14 (2017). WB ; Rat . Read more (PubMed: 28290154) »
- Li F et al. Ginsenoside Rg1 prevents starvation-induced muscle protein degradation via regulation of AKT/mTOR/FoxO signaling in C2C12 myotubes. Exp Ther Med 14:1241-1247 (2017). WB ; Mouse . Read more (PubMed: 28781621) »
客户评价及客户问答
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