Synthetic peptide containing a sequence corresponding to a region within amino acids 646-708 (PAKYQPYKKR GLCSRTACGF DFCVLCLCAY HGSEECSRGA AKPRNRKDAL PGSAQSKRNL KRL) of Human Fbp5B (NP_001025031).
293T whole cell lysate; Saos-2; A431, H1299, HeLa, HepG2, Molt-4 and Raji cell lysates
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 1X PBS, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 78 kDa.
1/100 - 1/250.
Members of the F-box protein family, such as Fbp5B, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).
Early mitotic inhibitor 2 antibody
Western blot - Fbp5B antibody (ab96858)
Anti-Fbp5B antibody (ab96858) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 78 kDa 7.5% SDS-PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fbp5B antibody (ab96858)
Immunohistochemical analysis of Fbp5B in paraffin-embedded Saos-2, using ab96858 at 1/100 dilution.
has not yet been referenced specifically in any publications.
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