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概述

  • 产品名称
    Anti-Fbp5B抗体
    参阅全部 Fbp5B 一抗
  • 描述
    兔多克隆抗体to Fbp5B
  • 特异性
    This antibody recognises an in vitro translation of Fbp5b. It also recognises a single slightly larger band in 293T cells that is reduced by peptide blocking. We believe this is strong evidence that the antibody is specifically recognising Fbp5b.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    SPPIVNSKYSTFRDFC

    , corresponding to amino acids 53 - 68 of Human Fbp5B.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 75 kDa).

靶标

  • 相关性
    Members of the F-box protein family, such as Fbp5B, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).
  • 数据库链接
    • 别名
      • FBXO43 antibody
      • Early mitotic inhibitor 2 antibody
      • EMI2 antibody
      • Endogenous meiotic inhibitor 2 antibody
      • ERP1 antibody
      • F box protein 43 antibody
      • Fbx43 antibody
      see all

    Anti-Fbp5B antibody 图像

    • Western blot - Anti-Fbp5B antibody (ab4506)
      Western blot - Anti-Fbp5B antibody (ab4506)This image is courtesy of Daniele Guardavaccaro, NYU Cancer Institute


      Predicted band size : 75 kDa

      This image is courtesy of Daniele Guardavaccaro, NYU Cancer Institute

      Western blot using ab4506.

      In panels A and B, there are two identical membranes (with an in
      vitro translated Fbp5b (Fbp5b ivt) and a whole cell extract from 293T cells loaded in duplicate) without  (A) and with (B) peptide blocking.

      Peptide inhibition completely removes the in vitro translated band and very strongly reduces the endogenous band from 293T cells.

      In a repeat of this experiment a mock in vitro translation was included and no band was seen in that lane.

      Western blot using ab4506.

      In panels A and B, there are two identical membranes (with an in vitro translated Fbp5b (Fbp5b ivt) and a whole cell extract from 293T cells loaded in duplicate) without (A) and with (B) peptide blocking.

      Peptide inhibition completely removes the in vitro translated band and very strongly reduces the endogenous band from 293T cells.

      In a repeat of this experiment a mock in vitro translation was included and no band wa

    实验方案

    Anti-Fbp5B antibody (ab4506)参考文献

    ab4506 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab4506？请让我们知道，以便我们可以引用本数据表中的参考文章。

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