Synthetic peptide:
SPPIVNSKYSTFRDFC
, corresponding to amino acids 53 - 68 of Human Fbp5B.Our Abpromise guarantee covers the use of ab4506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 75 kDa). |
Western blot using ab4506.
In panels A and B, there are two identical membranes (with an in
vitro translated Fbp5b (Fbp5b ivt) and a whole cell extract from 293T cells loaded in duplicate) without (A) and with (B) peptide blocking.
Peptide inhibition completely removes the in vitro translated band and very strongly reduces the endogenous band from 293T cells.
In a repeat of this experiment a mock in vitro translation was included and no band was seen in that lane.
Western blot using ab4506.ab4506 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"