概述
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产品名称Anti-Fbp5B抗体
参阅全部 Fbp5B 一抗 -
描述兔多克隆抗体to Fbp5B
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宿主Rabbit
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特异性This antibody recognises an in vitro translation of Fbp5b. It also recognises a single slightly larger band in 293T cells that is reduced by peptide blocking. We believe this is strong evidence that the antibody is specifically recognising Fbp5b.
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经测试应用适用于: WBmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide:
SPPIVNSKYSTFRDFC
, corresponding to amino acids 53 - 68 of Human Fbp5B.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab4506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 75 kDa). |
靶标
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相关性Members of the F-box protein family, such as Fbp5B, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).
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数据库链接
- Entrez Gene: 286151 Human
- Omim: 609110 Human
- SwissProt: Q4G163 Human
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别名
- FBXO43 antibody
- Early mitotic inhibitor 2 antibody
- EMI2 antibody
see all
图片
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Western blot - Anti-Fbp5B antibody (ab4506)This image is courtesy of Daniele Guardavaccaro, NYU Cancer InstituteWestern blot using ab4506.
In panels A and B, there are two identical membranes (with an in
vitro translated Fbp5b (Fbp5b ivt) and a whole cell extract from 293T cells loaded in duplicate) without (A) and with (B) peptide blocking.Peptide inhibition completely removes the in vitro translated band and very strongly reduces the endogenous band from 293T cells.
In a repeat of this experiment a mock in vitro translation was included and no band was seen in that lane.
Western blot using ab4506.
In panels A and B, there are two identical membranes (with an in vitro translated Fbp5b (Fbp5b ivt) and a whole cell extract from 293T cells loaded in duplicate) without (A) and with (B) peptide blocking.
Peptide inhibition completely removes the in vitro translated band and very strongly reduces the endogenous band from 293T cells.
In a repeat of this experiment a mock in vitro translation was included and no band wa
文献
ab4506 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"