重组
RabMAb

Anti-FBP1抗体[EPR4620] (ab109732)

概述

  • 产品名称
    Anti-FBP1抗体[EPR4620]
    参阅全部 FBP1 一抗
  • 描述
    兔单克隆抗体[EPR4620] to FBP1
  • 经测试应用
    适用于: ICC/IF, WB, IP, ICCmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human FBP1.

  • 阳性对照
    • Human stomach, Human fetal liver, and MCF7 lysates IF/ICC: HeLa cell line
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-FBP1 antibody (Alexa Fluor® 488) [EPR4620] (ab206716)

    Anti-FBP1 antibody (Alexa Fluor® 647) [EPR4620] (ab207051)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

应用

Our Abpromise guarantee covers the use of ab109732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 通路
      Carbohydrate biosynthesis; gluconeogenesis.
    • 疾病相关
      Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
    • 序列相似性
      Belongs to the FBPase class 1 family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 6-bisphosphatase 1 antibody
      • 6-bisphosphate 1-phosphohydrolase 1 antibody
      • D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
      • D-fructose-1 antibody
      • EC 3.1.3.11 antibody
      • F16P1_HUMAN antibody
      • FBP antibody
      • FBP 1 antibody
      • FBP1 antibody
      • FBPase 1 antibody
      • Fructose 1 6 bisphosphatase 1 antibody
      • Fructose bisphosphatase 1 antibody
      • Fructose-1 antibody
      • Growth inhibiting protein 17 antibody
      • Liver fructose bisphosphatase antibody
      see all

    图片

    • ICC/IF image of ab109732 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109732, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution

      Lane 1 : Human stomach lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : MCF7 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa

    文献

    This product has been referenced in:
    • Li N  et al. Clinical and Molecular Characterization of Patients with Fructose 1,6-Bisphosphatase Deficiency. Int J Mol Sci 18:N/A (2017). WB ; Monkey . Read more (PubMed: 28420223) »
    • Du Y  et al. Lysine malonylation is elevated in type 2 diabetic mouse models and enriched in metabolic associated proteins. Mol Cell Proteomics 14:227-36 (2015). Read more (PubMed: 25418362) »

    See all 3 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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