KLH conjugated synthetic peptide selected from the N-terminal region of human FAU.
- 293 cell lysate or human colon carcinoma tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium Azide
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Protein G purified
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/100 - 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
||1/50 - 1/100.
Anti-FAU antibody 图像
Western blot - FAU antibody (ab63063)
All lanes :
Anti-FAU antibody (ab63063) at 1/100 dilutionLane 1 :
293 cell lysate, nontransfectedLane 2 :
293 cell lysate, transiently transfected with the FAU gene
Lysates/proteins at 2 µg per lane.Predicted band size :
14 kDaObserved band size :
17 kDa (why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAU antibody (ab63063)
Ab63063 (1/50-1/100) staining human FAU in human colon carcinoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
Ab63063 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Anti-FAU antibody (ab63063)参考文献
has not yet been referenced specifically in any publications.
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