概述

  • 产品名称Anti-FAU抗体
    参阅全部 FAU 一抗
  • 描述
    小鼠多克隆抗体to FAU
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fusion protein containing the full length FAU precursor protein (Human)

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab43581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 14 kDa.

14kDa is the predicted molecular weight of the unprocessed FAU precursor protein consisting of the ubiquitin-like protein Fubi at the N terminus and ribosomal protein S30 at the C terminus.

靶标

  • 序列相似性Belongs to the ubiquitin family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 40S ribosomal protein S30 antibody
    • asr1 antibody
    • FAU antibody
    • FAU encoded ubiquitin like protein antibody
    • FAU1 antibody
    • FBR MuSV associated ubiquitously expressed antibody
    • Finkel Biskis Reilly murine sarcoma virus (FBR MuSV) ubiquitously expressed (fox derived) antibody
    • Finkel Biskis Reilly murine sarcoma virus (FBR MuSV) ubiquitously expressed antibody
    • Finkel Biskis Reilly murine sarcoma virus ubiquitously expressed antibody
    • FLJ22986 antibody
    • Fub1 antibody
    • Fubi antibody
    • MNSFbeta antibody
    • Monoclonal nonspecific suppressor factor beta antibody
    • Ribosomal protein S30 antibody
    • RPS30 antibody
    • S30 antibody
    • UBIM_HUMAN antibody
    • Ubiquitin like protein fubi and ribosomal protein S30 antibody
    • Ubiquitin like protein fubi antibody
    • Ubiquitin like S30 fusion protein antibody
    • Ubiquitin-like protein FUBI antibody
    see all

Anti-FAU antibody 图像

  • All lanes : Anti-FAU antibody (ab43581) at 1/1000 dilution

    Lane 1 : Total protein extract from E.coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen
    Lane 2 : Total protein extract from E.coli with ~50ng to 500ng of the antigen fusion protein

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa

Anti-FAU antibody (ab43581)参考文献

ab43581 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 7.5 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Treatment 4-12% bis-Tris
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jul 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (U2OS)
Specification U2OS
Permeabilization Yes - Triton-X
Fixative Paraformaldehyde
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提交于 Jul 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"