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Anti-Fatty Acid Synthase抗体(ab22759)

概述

  • 产品名称
    Anti-Fatty Acid Synthase抗体
    参阅全部 Fatty Acid Synthase 一抗
  • 描述
    兔多克隆抗体to Fatty Acid Synthase
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, IHC (PFA fixed), IHC-Fr, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 2450 to the C-terminus of Mouse Fatty Acid Synthase.

    (Peptide available as ab25719.)

  • 阳性对照
    • WB: 3T3-L1 nuclear lysate (ab14632), mouse brain (ab27253), mouse liver whole cell lysate (ab7935), A549, HAP1 and human liver lysates. ICC/IF: HEK293 cells.

应用

Our Abpromise guarantee covers the use of ab22759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 273 kDa (predicted molecular weight: 273 kDa).
IHC-P Use at an assay dependent concentration.
IHC (PFA fixed) Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 21098489

靶标

  • 功能
    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
  • 组织特异性
    Ubiquitous. Prominent expression in brain, lung, and liver.
  • 序列相似性
    Contains 1 acyl carrier domain.
  • 细胞定位
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

图片



  • Predicted band size : 273 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: Hu liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab22759 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab22759 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab22759 and ab18058 (loading control to Vinculin) were diluted at 1 µg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • ab22759 staining Fatty Acid Synthase in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 30 minutes at 23°C; antigen retrieval was by heat mediation in Citra Plus solution. Samples were incubated with primary antibody (2μg/ml) for 14 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab22759 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab22759, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions.

    The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-Fatty Acid Synthase antibody (ab22759) at 1 µg/ml

    Lane 1 : 3T3-L1 nuclear extract lysate (ab14632)
    Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 3 : Liver (Mouse) Tissue Lysate (ab7935)
    Lane 4 : 3T3-L1 nuclear extract lysate (ab14632) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml
    Lane 5 : Brain (Mouse) Tissue Lysate (ab27253) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml
    Lane 6 : Liver (Mouse) Tissue Lysate (ab7935) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 273 kDa
    Observed band size : 273 kDa
    Additional bands at : 100 kDa,35 kDa,50 kDa (possible IgG). We are unsure as to the identity of these extra bands.
  • Immunofluorescent staining for Fatty Acid Synthase in the rat striatum using Rabbit polyclonal to Fatty Acid Synthase (ab22759). Abundant staining was observed in the Striatum with lower levels of staining observed in the Corpus callosum. This is a montage of three pictures aquired using a X10 objective. ab22759 was used at 1/200 (2µg/ml) incubated overnight at room temperature.

    Secondary antibody used was anti-rabbit Alexa Fluor® 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.

文献

This product has been referenced in:
  • Stein S  et al. Impaired SUMOylation of nuclear receptor LRH-1 promotes nonalcoholic fatty liver disease. J Clin Invest 127:583-592 (2017). Read more (PubMed: 28094767) »
  • Choi MS  et al. S-nitrosylation of fatty acid synthase regulates its activity through dimerization. J Lipid Res 57:607-15 (2016). Read more (PubMed: 26851298) »

See all 24 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (LIVER)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
LIVER
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citra Plus
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T, HepG2, MEF)
Loading amount
20 µg
Specification
293T, HepG2, MEF
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

提交于 Jan 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
35 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Mr. Ahmed Nayef

Verified customer

提交于 Dec 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (C2C12)
Specification
C2C12
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat liver tissue)
Loading amount
140 µg
Specification
Rat liver tissue
Gel Running Conditions
Reduced Denaturing (6% gel. Sample was heated in laemli buffer at 70 C for 10 min before loading)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Smitha Koshy

Verified customer

提交于 Aug 13 2012

Thank you for your email.

We have not tested ab22759 with pig samples at this time, but I aligned the immunogen sequence against the pig sequence (UniProt accession linked below, in case the customer is wondering what sequence was used) and f...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (C2C12)
Total protein in input
200 µg
Specification
C2C12
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

提交于 Mar 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (muscle)
Loading amount
20 µg
Specification
muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
40 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 02 2011

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