Fatty Acid Oxidation Assay试剂盒(flow cytometry) (ab118183)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Fatty Acid Oxidation Assay试剂盒(flow cytometry)
参阅全部 Fatty Acid Oxidation 试剂盒 -
检测方法
Fluorescent -
样品类型
Adherent cells, Suspension cells -
检测类型
Quantitative -
产品概述
Fatty Acid Oxidation Assay Kit ab118183 contains antibodies against key enzymes of the mitochondrial fatty acid oxidation pathway for flow cytometry. The assay combines the power of single cell analysis obtained from flow cytometry with the specificity of antibody-based immunostaining to quantify protein levels in cultured cells. Cells are harvested and fixed/permeabilized in suspension, targets of interest are detected indirectly with highly specific well-characterized monoclonal antibodies that are then labeled with fluorescent antibodies
This product was previously called Fatty Acid Oxidation Human Flow Cytometry Kit.
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说明
Spin down the contents of the antibody vials upon receipt of the kit. Store all components upright at 4°C. This kit is stable for at least 6 months from receipt.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Flow cytometer
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 96 tests 100X ACADM Primary Antibody 1 x 0.12ml 100X ACADVL Primary Antibody 1 x 0.12ml 100X HADHA Primary Antibody 1 x 0.12ml 100X Normal Mouse IgG 1 x 0.12ml 100X Triton X-100 1 x 1.25ml 10X Phosphate Buffered Saline 1 x 100ml 400X Tween-20 1 x 4ml Blocking Solution 1 x 25ml -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Hydrolysis
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别名
- Beta Oxidation
- Beta-Oxidation
图片
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After background subtraction, the ACADVL deficient cell line shows a 93% reduction in the level of the ACADVL protein.
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Expression levels of HADHA was determined in primary fibroblasts by flow cytometry. Blue: control cell line. Red: deficient cell lines extracted from patients with well characterized mutations in each of the enzymes HADHB:p[R61H];[R247H]. Black: Background fluorescence was determined with the supplied negative control.
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After background subtraction, the LCHAD deficient cell line shows a 50% reduction in the level of the HADHA protein.
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Expression levels of ACADM was determined in primary fibroblasts by flow cytometry. Blue: control cell line. Red: deficient cell lines extracted from patients with well characterized mutations in each of the enzymes ACADM:p[K604E] Black: Background fluorescence was determined with the supplied negative control.
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After background subtraction, the ACADM deficient cell line shows a 57% reduction in the level of the ACADM protein.
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Antibody specificity demonstrated by immunocytochemistry. Cells were processes with the Flow cytometry protocol as explained above. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab118183 被引用在 9 文献中.
- Dai W et al. PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism. Cancer Commun (Lond) 42:848-867 (2022). PubMed: 35904817
- Wang M et al. Programmed PPAR-α downregulation induces inflammaging by suppressing fatty acid catabolism in monocytes. iScience 24:102766 (2021). PubMed: 34286232
- Zhang Z et al. Corticosterone Enhances the AMPK-Mediated Immunosuppressive Phenotype of Testicular Macrophages During Uropathogenic Escherichia coli Induced Orchitis. Front Immunol 11:583276 (2020). PubMed: 33363533
- Kumar N et al. Assessment of temporal functional changes and miRNA profiling of human iPSC-derived cardiomyocytes. Sci Rep 9:13188 (2019). PubMed: 31515494
- Bartel K et al. Connecting lysosomes and mitochondria - a novel role for lipid metabolism in cancer cell death. Cell Commun Signal 17:87 (2019). PubMed: 31358011
- Singh KB et al. Prostate cancer chemoprevention by sulforaphane in a preclinical mouse model is associated with inhibition of fatty acid metabolism. Carcinogenesis 39:826-837 (2018). PubMed: 29668854
- Pascual G et al. Targeting metastasis-initiating cells through the fatty acid receptor CD36. Nature 541:41-45 (2017). PubMed: 27974793
- Tabe Y et al. Bone Marrow Adipocytes Facilitate Fatty Acid Oxidation Activating AMPK and a Transcriptional Network Supporting Survival of Acute Monocytic Leukemia Cells. Cancer Res 77:1453-1464 (2017). PubMed: 28108519
- Cook KL et al. Endoplasmic Reticulum Stress Protein GRP78 Modulates Lipid Metabolism to Control Drug Sensitivity and Antitumor Immunity in Breast Cancer. Cancer Res 76:5657-5670 (2016). PubMed: 27698188