Anti-Fatty Acid Binding Protein 5抗体(ab84028)

概述

  • 产品名称Anti-Fatty Acid Binding Protein 5抗体
    参阅全部 Fatty Acid Binding Protein 5 一抗
  • 描述
    兔多克隆抗体to Fatty Acid Binding Protein 5
  • 经测试应用适用于: ICC/IF, IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Sheep, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Fatty Acid Binding Protein 5.

    (Peptide available as ab94388.)

  • 阳性对照
    • This antibody gave a positive signal in the following human lysates: skin tissue; HeLa whole cell; HepG2 whole cell; A431 whole cell.

性能

应用

Our Abpromise guarantee covers the use of ab84028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).

靶标

  • 功能High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.
  • 组织特异性Keratinocytes; highly expressed in psoriatic skin.
  • 序列相似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • 结构域Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CFABP antibody
    • Cutaneous fatty acid binding protein antibody
    • DA11 antibody
    • Differentiation associated lipid binding protein LP2 antibody
    • E-FABP antibody
    • EFABP antibody
    • Epidermal-type fatty acid-binding protein antibody
    • FABP5 antibody
    • FABP5_HUMAN antibody
    • fatty acid binding protein 5 (psoriasis-associated) antibody
    • Fatty acid binding protein epidermal antibody
    • Fatty acid-binding protein 5 antibody
    • Fatty acid-binding protein, epidermal antibody
    • Keratinocyte lipid binding protein antibody
    • KFABP antibody
    • Klbp antibody
    • mal1 antibody
    • PA-FABP antibody
    • PAFABP antibody
    • Psoriasis associated fatty acid binding protein homolog antibody
    • Psoriasis-associated fatty acid-binding protein homolog antibody
    see all

Anti-Fatty Acid Binding Protein 5 antibody 图像

  • Fatty Acid Binding Protein 5 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84028.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 16kDa; Fatty Acid Binding Protein 5

  • All lanes : Anti-Fatty Acid Binding Protein 5 antibody (ab84028) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • ICC/IF image of ab84028 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84028, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, MCF-7 cells at 5µg/ml.

Anti-Fatty Acid Binding Protein 5 antibody (ab84028)参考文献

ab84028 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab84028.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"