• 产品名称Anti-FATP2抗体
    参阅全部 FATP2 一抗
  • 描述
    兔多克隆抗体to FATP2
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human SLC27A2.

    (Peptide available as ab96629.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human liver; Human kidney; Human placenta.



Our Abpromise guarantee covers the use of ab85801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • 功能Acyl-CoA synthetase probably involved in bile acid metabolism. Proposed to activate C27 precurors of bile acids to their CoA thioesters derivatives before side chain cleavage via peroxisomal beta-oxidation occurs. In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Does not utilize C24 bile acids as substrates. In vitro, also activates long- and branched-chain fatty acids and may have additional roles in fatty acid metabolism. May be involved in translocation of long-chain fatty acids (LFCA) across membranes.
  • 组织特异性Expressed in liver, kidney, placenta and pancreas.
  • 序列相似性Belongs to the ATP-dependent AMP-binding enzyme family.
  • 细胞定位Endoplasmic reticulum membrane. Peroxisome membrane. Peripheral membrane associated with the lumenal side of peroxisomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACSVL1 antibody
    • FACVL1 antibody
    • FATP 2 antibody
    • FATP-2 antibody
    • FATP2 antibody
    • Fatty acid coenzyme A ligase, very long chain 1 antibody
    • Fatty acid transport protein 2 antibody
    • Fatty-acid-coenzyme A ligase antibody
    • hFACVL1 antibody
    • HsT17226 antibody
    • Long chain fatty acid CoA ligase antibody
    • Long-chain-fatty-acid--CoA ligase antibody
    • S27A2_HUMAN antibody
    • Slc27a2 antibody
    • Solute carrier family 27 (fatty acid transporter), member 2 antibody
    • Solute carrier family 27 member 2 antibody
    • THCA CoA ligase antibody
    • THCA-CoA ligase antibody
    • Very long chain acyl CoA synthetase antibody
    • Very long chain fatty acid CoA ligase antibody
    • Very long chain fatty acid coenzyme A ligase 1 antibody
    • very long-chain 1 antibody
    • Very long-chain acyl-CoA synthetase antibody
    • Very long-chain-fatty-acid-CoA ligase antibody
    • VLACS antibody
    • VLCS antibody
    see all

Anti-FATP2 antibody 图像

  • All lanes : Anti-FATP2 antibody (ab85801) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 29 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab85801 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85389 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FATP2 antibody (ab85801)参考文献

ab85801 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Cow Tissue lysate - whole (Liver, muscle)
Loading amount 50 µg
Specification Liver, muscle
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

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提交于 Jun 21 2011