antigen sequence, corresponding to amino acids 3985-4092 of Human FAT3 (UniProt ID: Q8TDW7)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in the interactions between neurites derived from specific subsets of neurons during development.
Expressed in ES cells, primitive neuroectoderm, fetal brain, infant brain, adult neural tissues and prostate.
Contains 33 cadherin domains.
Contains 4 EGF-like domains.
Contains 1 laminin G-like domain.
Information by UniProt
- Cadherin family member 15 antibody
- CDHF15 antibody
- CDHR10 antibody
Immunocytochemistry/ Immunofluorescence - Anti-FAT3 antibody (ab121896)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121896 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAT3 antibody (ab121896)
ab121896 at a 1/10 dilution staining FAT3 in Human pancreas tissue (paraffin embedded) by immunohistochemistry.
has not yet been referenced specifically in any publications.
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