Immunofluorescent staining of FASTK in HeLa cells using ab97544 at a dilution of 1/1000. HeLa cells were fixed in ice-cold methanol for 5 minutes and the cells were counter-stained with Hoechst 33342.
Other - Anti-FASTK antibody (ab97544)Image from Zhi F et al., PLoS One. 2013;8(8):e72390. Fig 3.; doi: 10.1371/journal.pone.0072390
FASTK siRNA interference assay. Three siRNA sequences targeting different sites of human FASTK cDNA and a scrambled control siRNA (si-NC) were transfected into U251 cells using Lipofectamine 2000. Total protein or total RNA was isolated at 48 h or 24 h post-transfection. FASTK protein levels were determined by western blot analysis using ab97544 at a dilution of 1/500 (A). The secondary antibody was a rabbit IgG-HRP and the quantitive data was obtained with the Quantity One analysis program. FASTK mRNA levels were assessed by qRT-PCR (B).
Western blot - FASTK antibody (ab97544)
All lanes : Anti-FASTK antibody (ab97544) at 1/500 dilution
Lane 1 : MOLT4 whole cell lysate Lane 2 : Raji whole cell lysate