Recombinant protein fragment containing a sequence corresponding to a region within amino acids 396-521 of Human FASTK (NP_006703)
Our Abpromise guarantee covers the use of ab97544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/500 - 1/3000. Predicted molecular weight: 61 kDa. | |
ICC/IF | 1/100 - 1/200. |
Immunohistochemical staining of paraffin-embedded SCC15 xenograft, using ab97544 at 1/100 dilution.
Immunofluorescent staining of FASTK in HeLa cells using ab97544 at a dilution of 1/1000. HeLa cells were fixed in ice-cold methanol for 5 minutes and the cells were counter-stained with Hoechst 33342.
FASTK siRNA interference assay. Three siRNA sequences targeting different sites of human FASTK cDNA and a scrambled control siRNA (si-NC) were transfected into U251 cells using Lipofectamine 2000. Total protein or total RNA was isolated at 48 h or 24 h post-transfection. FASTK protein levels were determined by western blot analysis using ab97544 at a dilution of 1/500 (A). The secondary antibody was a rabbit IgG-HRP and the quantitive data was obtained with the Quantity One analysis program. FASTK mRNA levels were assessed by qRT-PCR (B).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"