- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-FASTK抗体
参阅全部 FASTK 一抗 -
描述兔多克隆抗体to FASTK
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宿主Rabbit
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Pig
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免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 396-521 of Human FASTK (NP_006703)
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阳性对照
- WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji or NIH3T3 cell lysates ICC/IF: HeLa cells
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... -
纯度Protein A purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab97544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 61 kDa. | |
| ICC/IF | 1/100 - 1/200. |
靶标
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功能Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor.
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组织特异性Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
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序列相似性Belongs to the FAST protein kinase family.
Contains 1 RAP domain. -
翻译后修饰Autophosphorylated on serine/threonine residues. Activated by dephosphorylation.
- Information by UniProt
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数据库链接
- Entrez Gene: 509781 Cow
- Entrez Gene: 482802 Dog
- Entrez Gene: 10922 Human
- Entrez Gene: 66587 Mouse
- Entrez Gene: 296741 Rat
- Omim: 606965 Human
- SwissProt: Q14296 Human
- SwissProt: Q9JIX9 Mouse
see all -
别名
- Fas activated serine threonine kinase antibody
- Fas-activated serine/threonine kinase antibody
- FAST antibody
see all
图片
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Western blot - Anti-FASTK antibody (ab97544)All lanes : Anti-FASTK antibody (ab97544) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa -
Western blot - Anti-FASTK antibody (ab97544)Anti-FASTK antibody (ab97544) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg
Predicted band size: 61 kDa -
Western blot - Anti-FASTK antibody (ab97544)Anti-FASTK antibody (ab97544) at 1/1000 dilution + NIH-3T3 cell lysate at 30 µg
Predicted band size: 61 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FASTK antibody (ab97544)Immunohistochemical staining of paraffin-embedded SCC15 xenograft, using ab97544 at 1/100 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-FASTK antibody (ab97544)Immunofluorescent staining of FASTK in HeLa cells using ab97544 at a dilution of 1/1000. HeLa cells were fixed in ice-cold methanol for 5 minutes and the cells were counter-stained with Hoechst 33342.
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Other - Anti-FASTK antibody (ab97544)Image from Zhi F et al., PLoS One. 2013;8(8):e72390. Fig 3.; doi: 10.1371/journal.pone.0072390FASTK siRNA interference assay. Three siRNA sequences targeting different sites of human FASTK cDNA and a scrambled control siRNA (si-NC) were transfected into U251 cells using Lipofectamine 2000. Total protein or total RNA was isolated at 48 h or 24 h post-transfection. FASTK protein levels were determined by western blot analysis using ab97544 at a dilution of 1/500 (A). The secondary antibody was a rabbit IgG-HRP and the quantitive data was obtained with the Quantity One analysis program. FASTK mRNA levels were assessed by qRT-PCR (B).
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Western blot - Anti-FASTK antibody (ab97544)All lanes : Anti-FASTK antibody (ab97544) at 1/500 dilution
Lane 1 : MOLT4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-FASTK antibody (ab97544)Immunofluorescence analysis of FASTK in methanol fixed HeLa, using ab97544 at a 1/200 dilution.
Lower image is co-stained with Hoechst 33342.
文献
This product has been referenced in:
- Zhi F et al. miR-106a-5p inhibits the proliferation and migration of astrocytoma cells and promotes apoptosis by targeting FASTK. PLoS One 8:e72390 (2013). WB . Read more (PubMed: 24013584) »
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