Anti-fast skeletal Myosin抗体[Y32], prediluted (ab909)

概述

  • 产品名称Anti-fast skeletal Myosin抗体[Y32], prediluted
    参阅全部 fast skeletal Myosin 一抗
  • 描述
    小鼠单克隆抗体[Y32] to fast skeletal Myosin, prediluted
  • 特异性This antibody recognizes heavy chain of human skeletal myosin. It does not stain cardiac or smooth muscle myosin. This antibody stains the fast twitch (type II) isomyosin molecule.
  • 经测试应用适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Human
  • 免疫原

    Full length protein (Rabbit).

  • 阳性对照
    • IHC-P: FFPE human skeletal msucle tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
IHC-Fr
  • 应用说明This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
  • 靶标

    Anti-fast skeletal Myosin antibody [Y32], prediluted 图像

    • IHC image of fast skeletal Myosin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab909, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

    Anti-fast skeletal Myosin antibody [Y32], prediluted (ab909)参考文献

    This product has been referenced in:
    • Haider KH  et al. MicroRNA-21 is a key determinant in IL-11/Stat3 anti-apoptotic signalling pathway in preconditioning of skeletal myoblasts. Cardiovasc Res 88:168-78 (2010). IHC-Fr ; Rat . Read more (PubMed: 20498256) »
    • Barker T  et al. Vitamin E and C supplementation does not ameliorate muscle dysfunction after anterior cruciate ligament surgery. Free Radic Biol Med 47:1611-8 (2009). IHC-P ; Human . Read more (PubMed: 19751822) »

    See all 2 Publications for this product

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