特异性This antibody recognises all fast myosin heavy chains (embryonic, neonatal, adult) in chicks but reacts with neonatal and adult rat/mouse myosin heavy chains showing no reaction with the embryonic/fetal isoform.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 223 kDa.
Use at an assay dependent dilution.
序列相似性Contains 1 IQ domain. Contains 1 myosin head-like domain.
结构域The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
细胞定位Cytoplasm > myofibril. Thick filaments of the myofibrils.