Anti-Fast Myosin Skeletal Heavy chain抗体(ab91506)


  • 产品名称Anti-Fast Myosin Skeletal Heavy chain抗体
    参阅全部 Fast Myosin Skeletal Heavy chain 一抗
  • 描述
    兔多克隆抗体to Fast Myosin Skeletal Heavy chain
  • 经测试应用适用于: IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
    预测可用于: Sheep, Guinea pig, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Fast Myosin Skeletal Heavy chain.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Skeletal Muscle (Human) Tissue Lysate; Skeletal Muscle (Mouse) Tissue Lysate; Skeletal Muscle (Rat) Tissue Lysate



Our Abpromise guarantee covers the use of ab91506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 23515448
WB Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P Use a concentration of 1 µg/ml.


Anti-Fast Myosin Skeletal Heavy chain antibody 图像

  • IHC image of Fast Myosin Skeletal Heavy chain staining in Mouse skeletal muscle FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • All lanes : Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 223 kDa

    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab91506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunohistochemical analysis of sheep muscle tissue frozen section, labeling Fast Myosin Skeletal Heavy Chain with ab91506. Samples were fixed in methanol, blocking was with 3% BSA for 30 minutes at 25°C. Samples were incubated with ab91506 diluted 1/200 for 16 hours at 4°C. 

    See Abreview

Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)参考文献

This product has been referenced in:
  • Ackermann KA  et al. Early changes of muscle membrane properties in porcine faecal peritonitis. Crit Care 18:484 (2014). WB ; Pig . Read more (PubMed: 25145497) »
  • Stuart CA  et al. Slow-twitch fiber proportion in skeletal muscle correlates with insulin responsiveness. J Clin Endocrinol Metab 98:2027-36 (2013). IHC-Fr ; Human . Read more (PubMed: 23515448) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Rat Cell (Muscle Tissue)
Permeabilization Yes - TritonX 0,1%
Specification Muscle Tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative Acetone

Abcam user community

Verified customer

提交于 Nov 02 2016

Application Immunohistochemistry (Frozen sections)
Sample Sheep Tissue sections (muscle)
Permeabilization No
Specification muscle
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Methanol

Abcam user community

Verified customer

提交于 Oct 10 2015