The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 23515448
Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
Use a concentration of 1 µg/ml.
序列相似性Contains 1 IQ domain. Contains 1 myosin head-like domain.
结构域The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
细胞定位Cytoplasm > myofibril. Thick filaments of the myofibrils.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fast Myosin Skeletal Heavy chain antibody (ab91506)
IHC image of Fast Myosin Skeletal Heavy chain staining in Mouse skeletal muscle FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Western blot - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
All lanes : Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/50000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 223 kDa Observed band size : 223 kDa
Exposure time : 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab91506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Immunohistochemistry (Frozen sections) - Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)This image is courtesy of an anonymous Abreview.
Immunohistochemical analysis of sheep muscle tissue frozen section, labeling Fast Myosin Skeletal Heavy Chain with ab91506. Samples were fixed in methanol, blocking was with 3% BSA for 30 minutes at 25°C. Samples were incubated with ab91506 diluted 1/200 for 16 hours at 4°C.