FITC荧光Anti-Fas抗体[LOB 3/17] (ab33227)
Key features and details
- FITC Mouse monoclonal [LOB 3/17] to Fas
- Suitable for: Flow Cyt
- Reacts with: Human, Rhesus monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
概述
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产品名称
FITC荧光Anti-Fas抗体[LOB 3/17]
参阅全部 Fas 一抗 -
描述
FITC荧光小鼠单克隆抗体[LOB 3/17] to Fas -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human, Rhesus monkey -
免疫原
Fusion protein corresponding to Human Fas.
Database link: P25445 -
阳性对照
- HUT78 T cell line.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
LOB 3/17 -
骨髓瘤
NS1 -
同种型
IgG1 -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33227于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
1/1 - 1/10.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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说明 |
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Flow Cyt
1/1 - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
靶标
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功能
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). -
组织特异性
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. -
疾病相关
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
序列相似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
结构域
Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 355 Human
- Omim: 134637 Human
- SwissProt: P25445 Human
- Unigene: 244139 Human
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别名
- ALPS 1A antibody
- ALPS1A antibody
- APO 1 antibody
see all
图片
数据表及文件
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Datasheet download
文献 (1)
ab33227 被引用在 1 文献中.
- Chen JY et al. TNF-alpha renders human peritoneal mesothelial cells sensitive to anti-Fas antibody-induced apoptosis. Nephrol Dial Transplant 18:1741-7 (2003). PubMed: 12937219