Anti-Fas抗体[DX2] (Allophycocyanin) (ab25290)

概述

  • 产品名称
    Anti-Fas抗体[DX2] (Allophycocyanin)
    参阅全部 Fas 一抗
  • 描述
    小鼠单克隆抗体[DX2] to Fas (Allophycocyanin)
  • 偶联物
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • 特异性
    ab25290 recognises CD95/Fas/Apo-1.
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 常规说明


    ab25290 can be used for in vitro induction of apoptosis.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

应用

Our Abpromise guarantee covers the use of ab25290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

In vitro induction of apoptosis.

靶标

  • 功能
    Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • 组织特异性
    Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • 疾病相关
    Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • 序列相似性
    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • 结构域
    Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALPS 1A antibody
    • ALPS1A antibody
    • APO 1 antibody
    • Apo 1 antigen antibody
    • APO 1 cell surface antigen antibody
    • Apo-1 antigen antibody
    • APO1 antibody
    • Apo1 antigen antibody
    • APO1 cell surface antigen antibody
    • Apoptosis antigen 1 antibody
    • Apoptosis mediating surface antigen FAS antibody
    • Apoptosis-mediating surface antigen FAS antibody
    • APT 1 antibody
    • APT1 antibody
    • CD 95 antibody
    • CD 95 antigen antibody
    • CD95 antibody
    • CD95 antigen antibody
    • Delta Fas antibody
    • Delta Fas/APO 1/CD95 antibody
    • Delta Fas/APO1/CD95 antibody
    • Fas (TNF receptor superfamily, member 6) antibody
    • FAS 1 antibody
    • FAS 827dupA antibody
    • Fas AMA antibody
    • Fas antibody
    • FAS Antigen antibody
    • Fas cell surface death receptor antibody
    • FAS1 antibody
    • FASLG receptor antibody
    • FASTM antibody
    • sFAS antibody
    • Surface antigen APO1 antibody
    • TNF receptor superfamily, member 6 antibody
    • TNFRSF 6 antibody
    • TNFRSF6 antibody
    • TNR6_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 6 antibody
    see all

图片

文献

ab25290 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: from spleen
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive cells
Username

Abcam user community

Verified customer

提交于 Jan 10 2013

Application
Flow Cytometry
Sample
Mouse Cell (splenocytes)
Specification
splenocytes
Preparation
Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive cells
Username

Abcam user community

Verified customer

提交于 Jan 02 2013

Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive cells
Username

Abcam user community

Verified customer

提交于 Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive cells
Username

Abcam user community

Verified customer

提交于 Jan 02 2013

Thank you for contacting us. ab19456, ab11881, ab34359, ab25405 and ab25290 antibodies can be used in Biological studies which may include apoptosis. We unfortunately haven’t tested these antibodies in our own laboratory so unfortunately we will no...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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