概述

  • 产品名称Anti-Fas抗体
    参阅全部 Fas 一抗
  • 描述
    兔多克隆抗体to Fas
  • 特异性Recognizes human, mouse and rat Fas. Detects a band of ~45kDa by Western blot.
  • 经测试应用适用于: ICC/IF, Flow Cyt, IHC-P, IHC-Fr, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Alligator
  • 免疫原

    Synthetic peptide (Mouse) derived from the extracellular domain, conjugated to KLH

  • 阳性对照
    • Rat and Mouse brain tissue extracs; Human hepatoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab82419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50.
IHC-Fr 1/50.
ICC 1/50.
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • 组织特异性Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • 疾病相关Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • 序列相似性Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • 结构域Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALPS 1A antibody
    • ALPS1A antibody
    • APO 1 antibody
    • Apo 1 antigen antibody
    • APO 1 cell surface antigen antibody
    • Apo-1 antigen antibody
    • APO1 antibody
    • Apo1 antigen antibody
    • APO1 cell surface antigen antibody
    • Apoptosis antigen 1 antibody
    • Apoptosis mediating surface antigen FAS antibody
    • Apoptosis-mediating surface antigen FAS antibody
    • APT 1 antibody
    • APT1 antibody
    • CD 95 antibody
    • CD 95 antigen antibody
    • CD95 antibody
    • CD95 antigen antibody
    • Delta Fas antibody
    • Delta Fas/APO 1/CD95 antibody
    • Delta Fas/APO1/CD95 antibody
    • Fas (TNF receptor superfamily, member 6) antibody
    • FAS 1 antibody
    • FAS 827dupA antibody
    • Fas AMA antibody
    • Fas antibody
    • FAS Antigen antibody
    • Fas cell surface death receptor antibody
    • FAS1 antibody
    • FASLG receptor antibody
    • FASTM antibody
    • sFAS antibody
    • Surface antigen APO1 antibody
    • TNF receptor superfamily, member 6 antibody
    • TNFRSF 6 antibody
    • TNFRSF6 antibody
    • TNR6_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 6 antibody
    see all

Anti-Fas antibody 图像

  • All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution

    Lane 1 : Rat brain tissue extract
    Lane 2 : Mouse brain tissue extract

    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • ab82419 staining CD95 in rat spleen tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in 4% paraformaldehyde, blocked with 10% donkey serum (ab7475) for 20 minutes at 24°C, then incubated with ab82419 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal (ab150073), used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).

    See Abreview

  • ab82419 staining CD95 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) ab150073) was used as the secondary antibody.

    See Abreview

Anti-Fas antibody (ab82419)参考文献

This product has been referenced in:
  • Wang J  et al. Mechanism of QSYQ on anti-apoptosis mediated by different subtypes of cyclooxygenase in AMI induced heart failure rats. BMC Complement Altern Med 15:352 (2015). WB ; Rat . Read more (PubMed: 26445960) »
  • Yeh CN  et al. Fas/Fas Ligand Mediates Keratinocyte Death in Sunitinib-Induced Hand-Foot Skin Reaction. J Invest Dermatol N/A:N/A (2014). Read more (PubMed: 24802849) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (spleen)
Specification spleen
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 17 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Tissue sections (Retina)
Specification Retina
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 17 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Mouse Tissue sections (Uterus)
Specification Uterus
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

提交于 Nov 12 2013

Thank you for your enquiry and your interest in our products.

I can confirm that ab82419 has been tested for these applications i.e. Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ICC, WB.

However, we have not characterized it for induction/funct...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (RAW267.4 cell line)
Specification RAW267.4 cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (Kelly Neuroblastoma cell line)
Specification Kelly Neuroblastoma cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cell (33B Cell line)
Specification 33B Cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (B50 Neuroblastoma cell line)
Specification B50 Neuroblastoma cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Macrophage RAW264.7 cell line)
Specification Macrophage RAW264.7 cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 May 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SH-SY5Y)
Specification SH-SY5Y
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Oct 07 2011

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"